Skip to main content Skip to navigation
FITC Mouse Anti-Rat CD59
FITC Mouse Anti-Rat CD59

Flow cytometric analysis of CD59 on rat bone marrow. Bone marrow from Lewis rat were stained with either FITC mouse IgG1, κ isotype control (Cat. No. 550616, dashed line histogram) or a FITC mouse anti-rat CD59 antibody (Cat.No. 550976, solid line histogram) in conjunction with a BD Horizon™ V450 mouse anti-rat CD45 antibody (Cat.No. 561587). Flow cytometric fluorescence histograms were derived from gated events with CD45 negative cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Flow cytometric analysis of CD59 on rat bone marrow. Bone marrow from Lewis rat were stained with either FITC mouse IgG1, κ isotype control (Cat. No. 550616, dashed line histogram) or a FITC mouse anti-rat CD59 antibody (Cat.No. 550976, solid line histogram) in conjunction with a BD Horizon™ V450 mouse anti-rat CD45 antibody (Cat.No. 561587). Flow cytometric fluorescence histograms were derived from gated events with CD45 negative cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
MACIF; Membrane attack complex inhibition factor; Protectin
Rat (QC Testing)
Mouse BALB/c IgG1, κ
Membrane attack complex-inhibitory proteins from Rat erythrocyte membranes
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_2076342
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. An isotype control should be used at the same concentration as the antibody of interest.
550976 Rev. 6
Antibody Details
Down Arrow Up Arrow
TH9

The TH9 antibody monoclonal antibody specifically binds to CD59, a 21 kDa glycosyl-phosphatidyl inositol-anchored cell-surface glycoprotein of the Ly-6 superfamily. CD59 is expressed by many types of non-hematopoietic cells. In the rat hematopoietic system, CD59 has been detected on erythrocytes, monocytes, and some lymphocytes, but not on platelets. Soluble CD59 is found in body fluids and urine. CD59 is a complement regulatory protein that acts late in the complement cascade to prevent formation of the membrane attack complex (MAC). Therefore, CD59 is one of several proteins whose function is to protect host tissue from complement attack. Rat CD59 binds rat and human complement components and inhibits cytolysis mediated by complement from multiple species. CD59 has also been suggested to be a ligand for CD2 and to participate in T-cell costimulation.

550976 Rev. 6
Format Details
Down Arrow Up Arrow
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
FITC
Blue 488 nm
494 nm
518 nm
550976 Rev.6
Citations & References
Down Arrow Up Arrow

Development References (6)

  1. Funabashi K, Okada N, Matsuo S, Yamamoto T, Morgan BP, Okada H. Tissue distribution of complement regulatory membrane proteins in rats. Immunology. 1994; 81(3):444-451. (Biology). View Reference
  2. Hughes TR, Piddlesden SJ, Williams JD, Harrison RA, Morgan BP. Isolation and characterization of a membrane protein from rat erythrocytes which inhibits lysis by the membrane attack complex of rat complement. Biochem J. 1992; 284(1):169-176. (Immunogen). View Reference
  3. Lehto T, Morgan BP, Meri S. Binding of human and rat CD59 to the terminal complement complexes. Immunology. 1997; 90(1):121-128. (Biology). View Reference
  4. Liversidge J, Dawson R, Hoey S, McKay D, Grabowski P, Forrester JV. CD59 and CD48 expressed by rat retinal pigment epithelial cells are major ligands for the CD2-mediated alternative pathway of T cell activation. J Immunol. 1996; 156(10):3696-3703. (Clone-specific: (Co)-stimulation). View Reference
  5. Rushmere NK, Tomlinson S, Morgan BP. Expression of rat CD59: functional analysis confirms lack of species selectivity and reveals that glycosylation is not required for function. Immunology. 1997; 90(4):640-646. (Biology). View Reference
  6. Sugita Y, Masuho Y. CD59: its role in complement regulation and potential for therapeutic use. Immunotechnology. 1995; 1(3-4):157-168. (Biology). View Reference
View All (6) View Less
550976 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.