BD Horizon™ BV421 Rat Anti-Mouse CD226 (DNAM-1)
Clone TX42.1 (also known as TX42) (RUO)
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
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Companion Products
The TX42.1 monoclonal antibody specifically recognizes CD226 which is also known as DNAX accessory molecule-1 (DNAM-1), Platelet and T-cell activation antigen 1 (Pta1), or T lineage-specific activation antigen 1 (TLiSA1). CD226 is an ~65 kDa type I transmembrane glycoprotein that is encoded by Cd226 (CD226 antigen) which belongs to the immunoglobulin superfamily (IgSF). The N-terminal extracellular region of CD226 (DNAM-1) contains two C2 type Ig-like domains which are followed by a transmembrane region and a cytoplasmic tail with predicted phosphorylation sites involved in signaling. This adhesion molecule is expressed on subsets of CD4+ and CD8+ T cells and B cells, NK cells, monocytes, macrophages, and platelets. CD226 (DNAM-1) associates with leukocyte function associated antigen-1 (LFA-1) and is involved in TCR-mediated signal transduction for T cell proliferation and differentiation. Interactions between the CD226 (DNAM-1) activating receptor and its ligands, CD112 and CD155, results in cellular signaling that promotes innate and adaptive immune responses, including the activation, differentiation and survival of cytotoxic cells.
Development References (5)
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Shibuya K, Shibata K, Tahara-Hanaoka S, Shibuya A. Comment on "CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions".. J Immunol. 2006; 176(7):3855; author reply 3856. (Clone-specific: Flow cytometry). View Reference
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Stanietsky N, Rovis TL, Glasner A, et al. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR.. Eur J Immunol. 2013; 43(8):2138-50. (Clone-specific: Flow cytometry). View Reference
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Tahara-Hanaoka S, Miyamoto A, Hara A, Honda S, Shibuya K, Shibuya A. Identification and characterization of murine DNAM-1 (CD226) and its poliovirus receptor family ligands.. Biochem Biophys Res Commun. 2005; 329(3):996-1000. (Immunogen: Blocking, Flow cytometry, Functional assay, Inhibition). View Reference
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Tahara-Hanaoka S, Shibuya K, Kai H, et al. Blood. 2006; 107(4):491-496. (Clone-specific: Blocking, Flow cytometry, Functional assay, In vivo exacerbation, Stimulation). View Reference
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Yamashita Y, Abe F, Hirochika R, Tahara-Hanaoka S, Shibuya A, Shibuya K. Establishment and characterization of a novel anti-DNAM-1 monoclonal antibody.. Monoclon Antib Immunodiagn Immunother. 2013; 32(1):60-4. (Clone-specific: In vivo exacerbation). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.