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      BV421 Mouse Anti-Biotin
      BV421 Mouse Anti-Biotin
      Multiparameter flow cytometric analysis of CD25 expression on viable Mouse splenic lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) and stained with Biotin Rat Anti-Mouse CD25 antibody (Cat. No. 567831). The cells were washed and then secondarily stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) or BD Horizon™ BV421 Mouse Anti-Biotin antibody (Cat. No. 570940/571012; Right Plot) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815) was added to cells right before analysis. The bivariate pseudocolor density plot showing CD25 expression (or Ig Isotype control staining) versus side-light scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      BV421 Mouse Anti-Biotin
      Multiparameter flow cytometric analysis of CD19 expression on viable Mouse splenic lymphocytes.    Left Panel - One-layer staining: Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142] and then stained with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) or BD Horizon™ BV421 Rat Anti-Mouse CD19 antibody (Cat. No. 562701; solid line histogram).    Right Panel - Two-layer staining: Mouse splenic leukocytes were preincubated with Mouse BD Fc Block™ and then stained with Biotin Rat Anti-Mouse CD19 antibody (Cat. No. 553784), washed, and secondarily stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Biotin antibody (Cat. No. 570940/571012; solid histogram) at 0.25 µg/test.    BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815) was added to cells right before analysis. The fluorescence histograms showing CD19 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      BV421 Mouse Anti-Biotin BV421 Mouse Anti-Biotin
      Multiparameter flow cytometric analysis of CD25 expression on viable Mouse splenic lymphocytes. Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) and stained with Biotin Rat Anti-Mouse CD25 antibody (Cat. No. 567831). The cells were washed and then secondarily stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) or BD Horizon™ BV421 Mouse Anti-Biotin antibody (Cat. No. 570940/571012; Right Plot) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815) was added to cells right before analysis. The bivariate pseudocolor density plot showing CD25 expression (or Ig Isotype control staining) versus side-light scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) splenic lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      Multiparameter flow cytometric analysis of CD19 expression on viable Mouse splenic lymphocytes.    Left Panel - One-layer staining: Mouse splenic leukocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553142] and then stained with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562602; dashed line histogram) or BD Horizon™ BV421 Rat Anti-Mouse CD19 antibody (Cat. No. 562701; solid line histogram).    Right Panel - Two-layer staining: Mouse splenic leukocytes were preincubated with Mouse BD Fc Block™ and then stained with Biotin Rat Anti-Mouse CD19 antibody (Cat. No. 553784), washed, and secondarily stained with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Biotin antibody (Cat. No. 570940/571012; solid histogram) at 0.25 µg/test.    BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815) was added to cells right before analysis. The fluorescence histograms showing CD19 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Horizon™
      Mouse IgG1, κ
      Biotin-conjugated Keyhole Limpet Hemocyanin (KLH)
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

         BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

         For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      8. For U.S. patents that may apply, see bd.com/patents.
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      570940 Rev. 1
      Antibody Details
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      BK-1/39

      The BK-1/39 monoclonal antibody specifically recognizes biotin. Biotin is a water-soluble B complex vitamin that functions as a coenzyme which is required for the cellular metabolism of proteins and fats and the production of fatty acids. Although required by all organisms, biotin synthesis is limited to bacteria, yeasts, molds, algae, and some plants. Biotin is also useful for tagging molecules such as nucleic acids or proteins including antibodies. The BK-1/39 antibody can used to detect biotinylated target molecules and antibodies especially when signal amplification is desired. Fluorescent BK-1/39 antibody conjugates can provide a useful alternative to fluorescent avidin conjugates in order to minimize background staining and maximize signal intensity.

      570940 Rev. 1
      Format Details
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      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      570940 Rev.1
      Citations & References
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      View product citations for antibody "570940" on CiteAb

      Development References (4)

      1. Levit-Zerdoun E, Becker M, Pohlmeyer R, et al. Survival of Igα-Deficient Mature B Cells Requires BAFF-R Function.. J Immunol. 2016; 196(5):2348-60. (Clone-specific: Flow cytometry). View Reference
      2. Mavrangelos C, Swart B, Nobbs S, Nicholson IC, Macardle PJ, Zola H. Detection of low-abundance membrane markers by immunofluorescence--a comparison of alternative high-sensitivity methods and reagents.. J Immunol Methods. 2004; 289(1-2):169-78. (Methodology: Flow cytometry). View Reference
      3. Pishesha N, Bilate AM, Wibowo MC, et al. Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease.. Proc Natl Acad Sci U S A. 2017; 114(12):3157-3162. (Clone-specific: Flow cytometry). View Reference
      4. Zempleni J, Wijeratne SS, Hassan YI. Biotin.. Biofactors. 2009; 35(1):36-46. (Biology). View Reference
      View All (4) View Less
      570940 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.