The rat anti-mouse IgE antibody (clone R35-72) reacts specifically with mouse IgE of the Igh-C [a] and Igh-C [b] haplotypes. It has been reported not to react with other Ig isotypes. Detection with the rat anti-mouse IgE antibody (clone R35-72) of surface immunoglobulin on IgE-secreting hybridoma cells has also been reported.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.