The V24-785 monoclonal antibody binds specifically to BDCA-2 (Blood Dendritic Cell Antigen 2), also known as CD303. As a member of the C-type lectin (CLEC) superfamily, it is an adhesion receptor that binds to galactose-terminated desialylated glycans on cell surfaces and on serum IgG. In the blood, BDCA-2 is expressed only on plasmacytoid dendritic cells (pDC). TLR-9 mediates the production of large amounts of IFN-α and IFN-β by activated pDC, and crosslinking of BDCA-2 inhibits that response.
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.