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Biotin Mouse Anti-Human BLTR-1(Leukotriene B4 Receptor)

Biotin Mouse Anti-Human BLTR-1(Leukotriene B4 Receptor)

Clone 203/14F11 (also known as 14F11)

(RUO)
Biotin Mouse Anti-Human BLTR-1(Leukotriene B4 Receptor)

Detection of BLTR-1 expression on human peripheral monocytes and granulocytes by biotinylated 203/14F11. Human lysed whole blood were stained with 20 µl/test of biotinylated 203/14F11. Streptavidin-PE (Cat. No. 554061) was used as second-step reagent. The data reflects gating on monocytes (left panel) and granulocytes (right panel) based on forward and side scattered light signals. The level of nonspecific staining was assessed by using biotinylated mouse IgG1 (Cat. No. 555747) as isotype control (shaded histogram).  

Detection of BLTR-1 expression on human peripheral monocytes and granulocytes by biotinylated 203/14F11. Human lysed whole blood were stained with 20 µl/test of biotinylated 203/14F11. Streptavidin-PE (Cat. No. 554061) was used as second-step reagent. The data reflects gating on monocytes (left panel) and granulocytes (right panel) based on forward and side scattered light signals. The level of nonspecific staining was assessed by using biotinylated mouse IgG1 (Cat. No. 555747) as isotype control (shaded histogram).  

Product Details
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BD Pharmingen™
hBLTR-1; BLT1; BLTR; P2Y7; GPR16; LTBR1; P2RY7; CMKRL1; LTB4R1
Human (QC Testing)
Mouse BALB/c IgG1
Human BLTR-1-transfected cell line
Flow cytometry (Routinely Tested)
20 µl
AB_394485
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
552835 Rev. 1
Antibody Details
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203/14F11

The monoclonal antibody 203/14F11 specifically recognizes human leukotriene B4 receptor 1 (BLTR-1). BLTR-1 is a seven-transmembrane, G-protein-coupled receptor. The receptor plays an important role in pro-inflammatory responses. Leukotrienne B4 receptors are distributed in various hematopoietic cells including monocytes, granulocytes, and lymphocytes. Leukotriene B4 (LTB4), a product  of arachidonic acid metabolism, is the ligand for BLTR-1. The immunogen used to generate 203/14F11 hybridoma was a human BLTR-1-transfected cell line.

552835 Rev. 1
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
552835 Rev.1
Citations & References
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Development References (3)

  1. Dasari VR, Jin J, Kunapuli SP. Distribution of leukotriene B4 receptors in human hematopoietic cells. Immunopharmacology. 2000; 48(2):157-163. (Biology). View Reference
  2. Pettersson A, Boketoft A, Sabirsh A, et al. First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1.. Biochem Biophys Res Commun. 2000; 279(2):520-5. (Immunogen). View Reference
  3. Yokomizo T, Masuda K, Kato K, Toda A, Izumi T, Shimizu T. Leukotriene B4 receptor. Cloning and intracellular signaling. Am J Respir Crit Care Med. 2000; 161(2):551-555. (Biology). View Reference
552835 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.