BD Horizon™ BB515 Mouse Anti-Mouse CD369 (CLEC7A)
Clone 2F3 (RUO)
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
For optimal results, it is recommended to perform 2 washes after staining with antibodies. Cells may be prepared, stained with antibodies and washed twice with wash buffer per established protocols for immunofluorescence staining, prior to acquisition on a flow cytometer. Performing fewer than the recommended wash steps may lead to increased spread of the negative population.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
The 2F3 monoclonal antibody specifically recognizes CD369 which is also known as Dectin-1 (Dendritic cell-associated C-type lectin 1) or BGR (Beta-glucan receptor). CD369 is a ~ 43 kDa type II transmembrane C-type lectin that is encoded by Clec7a (C-type lectin domain family 7, member a). The extracellular portion of CD369 (Clec7a) contains a C-terminal stalk with a carbohydrate recognition domain (CRD) that is followed by a transmembrane segment, and an ITAM-containing cytoplasmic tail. This protein is predominantly expressed on monocytes/macrophages, neutrophils and some dendritic cell populations and at a lower level on a sub-population of T cells. CD369 (Clec7a) binds to beta-glucan polymers and functions as a pattern recognition receptor (PRR) in innate immune response to fungal and bacterial pathogens. CD369 mediated signaling may play a role in leucocyte responses, including phagocytosis or enhanced cytokine production. In mice, two functionally different full-length and stalkless isoforms have been characterized. Both isoforms vary in their ability to recognize zymosan and mediate cellular responses upon zymosan recognition.
Development References (4)
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Brown GD, Taylor PR, Reid DM, et al. Dectin-1 is a major beta-glucan receptor on macrophages.. J Exp Med. 2002; 196(3):407-12. (Biology). View Reference
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Herre J, Gordon S, Brown GD. Dectin-1 and its role in the recognition of beta-glucans by macrophages.. Mol Immunol. 2004; 40(12):869-76. (Biology). View Reference
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Taylor PR, Brown GD, Reid DM, et al. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.. J Immunol. 2002; 169(7):3876-82. (Biology). View Reference
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Underhill DM, Rossnagle E, Lowell CA, Simmons RM. Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.. Blood. 2005; 106(7):2543-50. (Biology). View Reference
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