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      Alexa Fluor™ 647 Mouse Anti-Human Integrin αVβ6
      Alexa Fluor™ 647 Mouse Anti-Human Integrin αVβ6
      Flow cytometric analysis of Integrin αVβ6 expression on H441 cells.  Cells from the Human H441 (Papillary adenocarcinoma, ATCC® HTB-174™) cell line were stained with either Alexa Fluor™ 647 Mouse IgG2a, κ Isotype Control (Cat No. 565357; dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human Integrin αVβ6 antibody (Cat. No. 568396/568397; solid line histogram) at 1 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing Integrin αVβ6 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Alexa Fluor™ 647 Mouse Anti-Human Integrin αVβ6
      Flow cytometric analysis of Integrin αVβ6 expression on H441 cells.  Cells from the Human H441 (Papillary adenocarcinoma, ATCC® HTB-174™) cell line were stained with either Alexa Fluor™ 647 Mouse IgG2a, κ Isotype Control (Cat No. 565357; dashed line histogram) or Alexa Fluor™ 647 Mouse Anti-Human Integrin αVβ6 antibody (Cat. No. 568396/568397; solid line histogram) at 1 µg/test. DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing Integrin αVβ6 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry was performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      αVβ6; avb6; CD51/β6; ITGAV/ITGB6; Integrin alpha v beta 6
      Human (QC Testing), Mouse (Reported)
      Mouse 129 x C57BL/6 IgG2a
      129/C57BL/6 β6 -/- Mice were mmunized with Human αvβ6 or Mouse Keratinocytes
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      3685, 3694
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      5. An isotype control should be used at the same concentration as the antibody of interest.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      10. For U.S. patents that may apply, see bd.com/patents.
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      568397 Rev. 1
      Antibody Details
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      10D5

      The 10D5 monoclonal antibody specifically recognizes Integrin alpha v beta 6 (αvβ6) that is also known as CD51/β6. This heterodimeric receptor is comprised of transmembrane type I alpha and beta chain glycoproteins that are encoded by ITGAV (integrin subunit alpha) and ITGB6 (integrin subunit beta 6), respectively. Like other integrins, αvβ6 serves as a transmembrane adhesion receptor that can tether the intracellular cytoskeleton to the extracellular matrix (ECM) by binding to R-G-D sequences of ECM protein ligands including fibronectin, vitronectin, and tenascin. Integrin αvβ6 likewise plays a key role in the activation of transforming growth factor beta-1 (TGFβ1) by its R-G-D-dependent release of TGFβ1 from regulatory Latency-associated peptide (LAP). Some viruses, such as. Coxsackieviruses and Herpes simplex virus-1 use Integrin αvβ6 as a receptor to infect cells. Integrin αvβ6 engages in bidirectional signaling, ie, outside-in and inside-out signaling, to regulate essential cellular functions. These include adhesion, migration, polarization, differentiation, and proliferation that are crucial to tissue homeostasis. Although not normally expressed on adult epithelial cells/keratinocytes, its expression is upregulated on these cells during the course of embryonic development, inflammation, tissue injury, wound healing, as well as malignant transformation. The 10D5 antibody blocks Integrin αvβ6-dependent cellular adhesion and migration and reportedly recognizes both human and mouse αvβ6 integrins. Binding of 10D5 antibody to αvβ6 is divalent-cation dependent.

      568397 Rev. 1
      Format Details
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      Alexa Fluor™ 647
      Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      Alexa Fluor™ 647
      Red 627-640 nm
      653 nm
      669 nm
      568397 Rev.1
      Citations & References
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      View product citations for antibody "568397" on CiteAb

      Development References (14)

      1. Allen MD, Thomas GJ, Clark S, et al. Altered microenvironment promotes progression of preinvasive breast cancer: myoepithelial expression of αvβ6 integrin in DCIS identifies high-risk patients and predicts recurrence.. Clin Cancer Res. 2014; 20(2):344-57. (Clone-specific: Blocking, Flow cytometry, Functional assay). View Reference
      2. Bandyopadhyay A, Raghavan S. Defining the role of integrin alphavbeta6 in cancer.. Curr Drug Targets. 2009; 10(7):645-52. (Biology). View Reference
      3. Bates RC, Bellovin DI, Brown C, et al. Transcriptional activation of integrin beta6 during the epithelial-mesenchymal transition defines a novel prognostic indicator of aggressive colon carcinoma.. J Clin Invest. 2005; 115(2):339-47. (Clone-specific: Blocking). View Reference
      4. Huang X, Wu J, Spong S, Sheppard D. The integrin alphavbeta6 is critical for keratinocyte migration on both its known ligand, fibronectin, and on vitronectin.. J Cell Sci. 1998; 111 ( Pt 15):2189-95. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation, Inhibition). View Reference
      5. Jackson T, Sheppard D, Denyer M, Blakemore W, King AM. The epithelial integrin alphavbeta6 is a receptor for foot-and-mouth disease virus.. J Virol. 2000; 74(11):4949-56. (Clone-specific: Blocking). View Reference
      6. Jovanovic J, Takagi J, Choulier L, et al. alphaVbeta6 is a novel receptor for human fibrillin-1. Comparative studies of molecular determinants underlying integrin-rgd affinity and specificity.. J Biol Chem. 2007; 282(9):6743-51. (Clone-specific: Blocking, Flow cytometry). View Reference
      7. Koivisto L, Bi J, Häkkinen L, Larjava H. Integrin αvβ6: Structure, function and role in health and disease.. Int J Biochem Cell Biol. 2018; 99:186-196. (Biology). View Reference
      8. Li X, Yang Y, Hu Y, et al. Alphavbeta6-Fyn signaling promotes oral cancer progression.. J Biol Chem. 2003; 278(43):41646-53. (Clone-specific: Blocking). View Reference
      9. Maier S, Paulsson M, Hartmann U. The widely expressed extracellular matrix protein SMOC-2 promotes keratinocyte attachment and migration.. Exp Cell Res. 2008; 314(13):2477-87. (Clone-specific: Blocking). View Reference
      10. Miller LC, Blakemore W, Sheppard D, Atakilit A, King AM, Jackson T. Role of the cytoplasmic domain of the beta-subunit of integrin alpha(v)beta6 in infection by foot-and-mouth disease virus.. J Virol. 2001; 75(9):4158-64. (Clone-specific: Blocking). View Reference
      11. Munger JS, Huang X, Kawakatsu H, et al. The integrin alpha v beta 6 binds and activates latent TGF beta 1: a mechanism for regulating pulmonary inflammation and fibrosis.. Cell. 1999; 96(3):319-28. (Clone-specific: Blocking, Functional assay). View Reference
      12. Thomas GJ, Lewis MP, Whawell SA, et al. Expression of the alphavbeta6 integrin promotes migration and invasion in squamous carcinoma cells.. J Invest Dermatol. 2001; 117(1):67-73. (Clone-specific: Blocking). View Reference
      13. Thomas GJ, Nyström ML, Marshall JF. Alphavbeta6 integrin in wound healing and cancer of the oral cavity.. J Oral Pathol Med. 2006; 35(1):1-10. (Biology). View Reference
      14. Williams CH, Kajander T, Hyypiä T, Jackson T, Sheppard D, Stanway G. Integrin alpha v beta 6 is an RGD-dependent receptor for coxsackievirus A9.. J Virol. 2004; 78(13):6967-73. (Clone-specific). View Reference
      View All (14) View Less
      568397 Rev. 1

       

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      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.