BD Multitest™ Anti-Human CD45RA FITC/CD45RO PE/CD3 PerCP/CD4 APC
Description
The CD45RA antibody, clone L48, is derived from hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with low-buoyant– density human lymphocytes. The CD45RO antibody, clone UCHL-1, derived from hybridization of P3/NS-1/1-Ag4- 1 mouse myeloma cells with spleen cells from BALB/c mice immunized with an interleukin-2 (IL-2)-dependent human T-cell line. The CD3 antibody, clone SK7, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. The CD4 antibody, clone SK3, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. The CD45RA antibody recognizes a molecular weight of 220-kilodalton (kDa) isoform of the leucocyte common antigen (LCA). The CD45RA antigen is a member of the CD45 antigen family that also includes the CD45, CD45RB, and CD45RO antigens. The CD45RO antibody, a member of the same CD45 family as CD45RA, recognizes a 180-kDa isoform of the LCA. The CD45 antigen is a protein tyrosine phosphatase. The CD3 antibody recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa). The CD4 antibody recognizes an antigen that interacts with class II molecules of the major histocompatibility complex and is the primary receptor for the human immunodeficiency virus. The cytoplasmic portion of the antigen is associated with the protein tyrosine kinase p56lck. The CD4 antigen can regulate the function of the CD3 antigen/TCR complex. CD4 also reacts with monocytes/macrophages that have an antigen density lower than that on helper/inducer T lymphocytes.
Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| N/A | N/A | | IgG1, κ,IgG2a, κ,IgG1, κ,IgG1, κ | N/A |
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