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Purified Mouse Anti-Thrombin Receptor/PAR1
Purified Mouse Anti-Thrombin Receptor/PAR1

Western blot analysis of Thrombin Receptor/PAR1 on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243). Lane 1: 1:250, lane 2: 1; 500, lane 3: 1: 1000 dilution of the mouse anti- Thrombin Receptor/PAR1 antibody.

Western blot analysis of Thrombin Receptor/PAR1 on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243). Lane 1: 1:250, lane 2: 1; 500, lane 3: 1: 1000 dilution of the mouse anti- Thrombin Receptor/PAR1 antibody.

Product Details
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BD Transduction Laboratories™
Protease Activated Receptor-1 (PAR1)
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Human Thrombin Receptor/PAR1 aa. 33-153
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
47 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611522 Rev. 1
Antibody Details
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14/Thrombin Receptor

Thrombin is a serine protease that regulates the activity of many cell types including the activation of platelets after vascular injury. Thrombin functions are mediated by G-protein-coupled protease-activated receptors that are homologous to substance P and thyrotropin receptors. Thrombin receptors (PAR1, PAR3, and PAR4) are seven transmembrane domain proteins with large N-terminal exodomains that contain a thrombin cleavage site (LDPR/S). Thrombin binds to the exodomain and cleaves the peptide bond between Arg-41 and Ser-42. This unmasks a new N-terminus with the sequence SFLLRN that acts as a tethered ligand. SFLLRN binds to the body of the thrombin receptor leading to irreversable activation. Both phosphorylation of the thrombin receptor and internalization may uncouple downstream signaling. PAR1 protein is expressed in vascular endothelial cells, smooth muscle cells, and macrophages, while PAR1 mRNA is expressed at higher levels in neonatal rat brain and lower levels in skeletal muscle, liver, and kidney. Thus, G-protein signaling via thrombin receptors, like PAR1, may be important for a diverse array of cellular functions, such as platelet activation and neural development.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611522 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611522 Rev.1
Citations & References
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Development References (4)

  1. Coughlin SR. How the protease thrombin talks to cells. Proc Natl Acad Sci U S A. 1999; 96(20):11023-11027. (Biology). View Reference
  2. Nelken NA, Soifer SJ, O'Keefe J, Vu TK, Charo IF, Coughlin SR. Thrombin receptor expression in normal and atherosclerotic human arteries. Cell. 1992; 90(4):1614-1621. (Biology). View Reference
  3. Niclou S, Suidan HS, Brown-Luedi M, Monard D. Expression of the thrombin receptor mRNA in rat brain. Cell Mol Biol (Noisy-le-grand). 1994; 40(3):421-428. (Biology). View Reference
  4. Vu TK, Hung DT, Wheaton 'VI, Coughlin SR. Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. J Clin Invest. 1991; 64(6):1057-1068. (Biology). View Reference
View All (4) View Less
611522 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.