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Purified Mouse Anti-p95PKL/GIT
Purified Mouse Anti-p95PKL/GIT

Western blot analysis of p95PKL/GIT on a rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- p95PKL/GIT antibody.

Purified Mouse Anti-p95PKL/GIT

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Western blot analysis of p95PKL/GIT on a rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- p95PKL/GIT antibody.

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Product Details
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BD Transduction Laboratories™
Paxillin-Kinase Linker
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Chicken p95PKL aa. 140-472
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
95 kDa
250 µg/ml
AB_398910
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611388 Rev. 1
Antibody Details
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13/P95PKL

Paxillin is involved in the integration of growth factor- and adhesion-mediated signal transduction pathways at focal adhesion sites. The LD motif of paxillin has been implicated in its binding to FAK, vinculin, and paxillin-kinase linker (p95PKL). p95PKL couples paxillin to a protein complex containing p21 GTPase-activated kinase (PAK), Nck, and the guanine nucleotide exchange factor, PIX. The sequence of p95PKL, highly homologous to human GIT1 and GIT2, predicts a structure that contains an N-terminal ARF-GAP domain containing a Zn2+ finger, three ankyrin repeats, an EF hand domain, and two IQ motifs. p95PKL binds to both paxillin and PIX and colocalizes with paxillin at focal adhesion sites. Overexpression of a paxillin LD4 deletion mutant in neuroblastoma cells inhibits insulin-like growth factor-1 mediated lamellipodia formation. Microinjection of this mutant into NIH3T3 cells has been reported to decrease migration into a wound. These alterations in cell motility are thought to involve PAK-mediated regulation of the actin cytoskeleton, since the LD4 motif of paxillin is required for p95PKL-dependent recruitment of PAK/PIX complexes to paxillin-containing focal adhesions sites. This antibody has been reported to recognize GIT1 and GIT2 proteins.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611388 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611388 Rev.1
Citations & References
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Development References (2)

  1. Ku GM, Yablonski D, Manser E, Lim L, Weiss A. A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT. EMBO J. 2001; 20(3):457-465. (Biology: Western blot). View Reference
  2. Turner CE, Brown MC, Perrotta JA, et al. Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling. J Cell Biol. 1999; 145(4):851-863. (Immunogen). View Reference
611388 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.