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Purified Mouse Anti- Mre11
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human Mre11 aa. 3-194
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
81 kDa
250 µg/ml
AB_398888
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611366 Rev. 1
Antibody Details
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18/MRE11

Originally identified in S. cerevisiae, Rad50 is one of a group of genes, designated the Rad52 epistasis group, whose products mediate DNA double-stranded break (DSB) repair. Many of these genes, including Rad50, are conserved in humans and have a similar function to their S. cerevisiae counterparts. In yeast, a multiprotein complex of Rad50, Mre11, and XRS2 has been implicated in the nucleocytic processing of DSBs, in homologous recombination, in nonhomologous end joining, and in telomere maintenance. In humans, Rad50 and Mre11 complex with up to three additional proteins (95 kDa, 200 kDa, and 350 kDa). The 95 kDa species is thought to be human XRS2, although a separate report has identified it as Nibrin, the product of the gene mutated in Nijmegen breakage syndrome. The Rad50-Mre11-p95 complex possesses endonuclease and 3' to 5' exonuclease activity. Mre11 may provide both the endonuclease and exonuclease activity, but may also be involved in other functions related to this complex. Mre11 is ubiquitously expressed, with the highest expression in proliferating tissues. Thus, MRE11 may function in a multiprotein complex that has many roles during DNA maintenance and repair.

611366 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611366 Rev.1
Citations & References
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Development References (3)

  1. Haber JE. The many interfaces of Mre11. Cell. 1998; 95(5):583-586. (Biology). View Reference
  2. Paull TT, Gellert M. Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex. Genes Dev. 1999; 13(10):1276-1288. (Biology). View Reference
  3. Petrini JH, Walsh ME, DiMare C, Chen XN, Korenberg JR, Weaver DT. Isolation and characterization of the human MRE11 homologue. Genomics. 1995; 29(1):80-86. (Biology). View Reference
611366 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.