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Purified Mouse Anti-MKP2
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human, Mouse, Frog (Tested in Development)
Mouse IgG1
Rat MKP2 aa. 13-127
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
43 kDa
250 µg/ml
AB_398169
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610850 Rev. 1
Antibody Details
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48/MKP2

The mitogen activated protein (MAP) kinases mediate signal transduction pathways involved in cellular growth and differentiation. These MAP kinases, including ERK1 and ERK2, are activated by phosphorylation on Tyr and Thr by MEK (MAP and ERK Kinase). Regulation of the resulting physiological effects of MAP Kinase activation is affected in part by MAP Kinase phosphatases (MKPs). These phosphatases have dual specificity, dephosphorylating both the tyrosine and threonine residues on MAP kinases. MKP2, which is widely expressed in human tissues, specifically dephosphorylates activated ERKs and JNK. These phosphatases have overlapping substrate specificities for the three known families of MAP kinases - p38, ERK, and JNK. All of the known MKPs contain a highly conserved carboxyl-terminal catalytic domain flanked by two CH2 (CDC25 homology 2) domains.

610850 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610850 Rev.1
Citations & References
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Development References (3)

  1. Black EJ, Walker M, Clark W, MacLaren A, Gillespie DA. Cell transformation by v-Jun deactivates ERK MAP kinase signalling. Oncogene. 2002; 21(42):6540-6548. (Clone-specific: Western blot). View Reference
  2. Hirsch DD, Stork PJ. Mitogen-activated protein kinase phosphatases inactivate stress-activated protein kinase pathways in vivo. J Biol Chem. 1997; 272(7):4568-4575. (Biology). View Reference
  3. Misra-Press A, Rim CS, Yao H, Roberson MS, Stork PJ. A novel mitogen-activated protein kinase phosphatase. Structure, expression, and regulation. J Biol Chem. 1995; 270(24):14587-14596. (Biology). View Reference
610850 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.