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Purified Mouse Anti-mEPHX
Purified Mouse Anti-mEPHX

Western blot analysis of mEPHX on a WI-38 cell lysate (Human lung fibroblasts; ATCC CCL-75). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-mEPHX antibody.

Purified Mouse Anti-mEPHX

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Western blot analysis of mEPHX on a WI-38 cell lysate (Human lung fibroblasts; ATCC CCL-75). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-mEPHX antibody.

Immunofluorescence staining of NIH/3T3 cells (Mouse embryo fibroblast cells; ATCC CRL-1658).

Product Details
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BD Transduction Laboratories™
Microsomal Epoxide Hydrolase; mEH; EPHX
Human (QC Testing), Mouse, Rat, Chicken, Frog (Tested in Development)
Mouse IgG1
Human mEPHX aa. 13-125
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
46 kDa
250 µg/ml
AB_398894
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611372 Rev. 1
Antibody Details
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17/mEPHX

Microsomal epoxide hydrolase (mEPHX/mEH/EPHX) is a bifunctional membrane protein that plays a role in the metabolism of reactive epoxide intermediates and in hepatocyte uptake of bile acids.  mEPHX protein and nucleic acid sequence is evolutionarily conserved in human, rats, and rabbits and mEPHX protein expression is found in the embryo and multiple organs.  Cellular expression of mEPHX has been localized to both the ER and sinusoidal plasma membrane.  In the ER, mEPHX is expressed in two topological orientations.  The type I orientated (Ccyt/Nexo) mEPHX remains in the ER, while the type II orientated (Cexo/Ncyto) mEPHX is localized to the plasma membrane.  mEPHX metabolizes DMBA to both inert metabolites and metabolites that are electrophilic and possibly carcinogenic.  mEPHX null-mice have been shown to be highly resistant to DMBA-induced carcinogenesis using skin cancer bioassays.  Thus, mEPHX may have protective effects, such as bile acid uptake and reactive epoxide metabolism, as well as harmful effects related to the production of carcinogens.

611372 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611372 Rev.1
Citations & References
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Development References (3)

  1. Hassett C, Aicher L, Sidhu JS, Omiecinski CJ. Human microsomal epoxide hydrolase: genetic polymorphism and functional expression in vitro of amino acid variants. Hum Mol Genet. 1994; 3(3):421-428. (Biology). View Reference
  2. Miyata M, Kudo G, Lee YH, Yang TJ. Targeted disruption of the microsomal epoxide hydrolase gene. Microsomal epoxide hydrolase is required for the carcinogenic activity of 7,12-dimethylbenz[a]anthracene. J Biol Chem. 1999; 274(34):23963-23968. (Biology). View Reference
  3. Zhu Q, von Dippe P, Xing W, Levy D. Membrane topology and cell surface targeting of microsomal epoxide hydrolase. Evidence for multiple topological orientations. J Biol Chem. 1999; 274(39):27898-27904. (Biology). View Reference
611372 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.