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Purified Mouse Anti-Human LSP-1
Purified Mouse Anti-Human LSP-1

Western blot analysis of LSP-1 on a EB1 cell lysate (Human Burkitt's Lymphoma; ATCC HTB-60™). Lane 1: 1:125, lane 2: 1:250, lane 3: 1:500 dilution of the mouse anti-human LSP-1 antibody.

Purified Mouse Anti-Human LSP-1

Immunofluorescence staining of Jurkat cells (Human T-cell leukemia; ATCC TIB-152™).

Western blot analysis of LSP-1 on a EB1 cell lysate (Human Burkitt's Lymphoma; ATCC HTB-60™). Lane 1: 1:125, lane 2: 1:250, lane 3: 1:500 dilution of the mouse anti-human LSP-1 antibody.

Immunofluorescence staining of Jurkat cells (Human T-cell leukemia; ATCC TIB-152™).

Product Details
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BD Transduction Laboratories™
Lymphocyte Specific Protein-1
Human (QC Testing)
Mouse IgG1
Human LSP-1 aa. 75-215
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
52 kDa
250 µg/ml
AB_398057
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/resources/cellbiology/index.jsp

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610734 Rev. 2
Antibody Details
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16/LSP-1

LSP-1 (Lymphocyte Specific Protein-1) is expressed in lymphocytes (B cells, cytotoxic T cells, and helper T cells), neutrophils, and macrophages and is involved in lymphocyte signal transduction.  LSP-1 is a cytoplasmic membrane-bound protein that, following activation, translocates to the cytosol.  This activation and translocation is mediated by phosphorylation of serine and threonine residues in the C-terminus of LSP-1 in response to concanavalin A or phorbal myristate acetate stimulation of murine T cells.  LSP-1 contains two N-terminal calcium-binding sites and has also been shown to bind to actin.  These results suggest that LSP-1 may be involved in the binding of actin to the plasma membrane following stimulation of PKC in murine T cells.  The mRNA is constitutively expressed; however, the protein is only expressed following activation of the cell.  Furthermore, LSP-1 is a substrate for MAPKAP kinase 2 (MAP kinase-activated protein kinase 2) in neutrophils.

610734 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610734 Rev.2
Citations & References
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Development References (5)

  1. Howard T, Li Y, Torres M, Guerrero A, Coates T. The 47-kD protein increased in neutrophil actin dysfunction with 47- and 89-kD protein abnormalities is lymphocyte-specific protein. Blood. 1994; 83(1):231-241. (Biology). View Reference
  2. Jongstra-Bilen J, Young AJ, Chong R, Jongstra J. Human and mouse LSP1 genes code for highly conserved phosphoproteins. J Immunol. 1990; 144(3):1104-1110. (Biology). View Reference
  3. Kadiyala RK, McIntyre BW, Krensky AM. Molecular cloning and characterization of WP34, a phosphorylated human lymphocyte differentiation and activation antigen. Eur J Immunol. 1990; 20(11):2417-2423. (Biology). View Reference
  4. Klein DP, Jongstra-Bilen J, Ogryzlo K, Chong R, Jongstra J. Lymphocyte-specific Ca2+-binding protein LSP1 is associated with the cytoplasmic face of the plasma membrane. Mol Cell Biol. 1989; 9(7):3043-3048. (Biology). View Reference
  5. Matsumoto N, Kojima S, Osawa T, Toyoshima S. Protein kinase C phosphorylates p50 LSP1 and induces translocation of p50 LSP1 in T lymphocytes. J Biochem (Tokyo). 1994; 117(1):222-229. (Biology). View Reference
View All (5) View Less
610734 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.