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Purified Mouse Anti-Human ISGF3γ
Purified Mouse Anti-Human ISGF3γ

Western blot analysis of ISGF3γ on a human endothelial cell lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human ISGF3γ antibody.

Purified Mouse Anti-Human ISGF3γ

Immunofluorescence staining of human fibroblasts.

Western blot analysis of ISGF3γ on a human endothelial cell lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human ISGF3γ antibody.

Immunofluorescence staining of human fibroblasts.

Product Details
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BD Transduction Laboratories™
Interferon Stimulated Gene Factor-3
Human (QC Testing)
Mouse IgG1
Human ISGF3γ (p48) aa.126-351
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
48 kDa
250 µg/ml
AB_397680
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610285 Rev. 1
Antibody Details
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6/ISGF3γ

ISGF3γ is a nuclear DNA-binding protein. ISGF3 (Interferon Stimulated Gene Factor-3) is the primary transcription activator induced by the binding of interferon α (IFNα) to cell surface receptors. The functional ISGF3 is a complex of four polypeptides of 113 kDa (Stat2), 91 kDa (Stat1α), 84 kDa (Stat1β), and 48 kDa. The first three polypeptides (also known as ISGF3γ) each contain SH2 and SH3 domains. In response to either IFNα, IFNγ, EGF, PDGF, or CSF-1 binding their respective receptors, the cytoplasmic ISGF3α subunits are tyrosine-phosphorylated and translocated to the nucleus where they form an active complex with the 48 kDa protein (ISGF3γ). This complex is responsible for modulating the transcription of interferon-stimulated genes (ISGs). Activated ISGF3α stabilizes ISGF3γ-DNA interactions. This results in a ternary protein-DNA complex with a 25-fold greater stability than ISGF3γ-DNA. Thus, growth factors and cytokines activate a common signal transduction pathway that leads to the phosphorylation and nuclear translocation of a group of latent cytoplasmic transcription factors.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (3)

  1. Dumler I, Kopmann A, Wagner K. Urokinase induces activation and formation of Stat4 and Stat1-Stat2 complexes in human vascular smooth muscle cells. J Biol Chem. 1999; 274(34):24059-24065. (Biology: Western blot). View Reference
  2. Köster M, Hauser H. Dynamic redistribution of STAT1 protein in IFN signaling visualized by GFP fusion proteins. Eur J Biochem. 1999; 260(1):137-144. (Biology: Immunofluorescence). View Reference
  3. Veals SA, Schindler C, Leonard D. Subunit of an alpha-interferon-responsive transcription factor is related to interferon regulatory factor and Myb families of DNA-binding proteins. Mol Cell Biol. 1992; 12(8):3315-3324. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.