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Purified Mouse Anti-Human DNA-PKcs
Purified Mouse Anti-Human DNA-PKcs

Western blot analysis of DNA-PKcs on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human DNA-PKcs antibody.

Western blot analysis of DNA-PKcs on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human DNA-PKcs antibody.

Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG2a
Human DNA-PKcs aa. 874-1024
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
465 kDa
250 µg/ml
AB_398123
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610804 Rev. 2
Antibody Details
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6/DNA-PKcs (p350)

DNA-PK is a trimeric enzyme that is composed of a catalytic subunit of ~ 465 kDa (DNA-PKcs) and a heterodimeric regulatory subunit of 70 kDa and 86 kDa. DNA-PKcs has been reported to be inactive alone and depends on the regulatory subunit for subcellular localization and kinase activity. DNA-PKcs belongs to the phosphatidylinositol (PI)3-kinase family and phosphorylates proteins, particularly transcription factors, but not lipids. It is most similar to the members of this family that regulate cell cycle control, DNA repair, and DNA damage. DNA-PKcs and Ku80 are involved in V(D)J recombination and DNA double-stranded break repair mechanisms. The immunodeficiency disorder SCID is the result of abnormal V(D)J recombination in T lymphocytes. In SCID mice, a portion of the DNA-PKcs C-terminal phosphatidylinositol 3-kinase domain is deleted due to a T to A nucleotide transversion that produces a premature stop codon and a truncated protein product. Thus, the mutated gene for DNA-PKcs may be the SCID gene.

610804 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610804 Rev.2
Citations & References
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Development References (4)

  1. Araki R, Fujimori A, Hamatani K. Nonsense mutation at Tyr-4046 in the DNA-dependent protein kinase catalytic subunit of severe combined immune deficiency mice. Proc Natl Acad Sci U S A. 1997; 94(6):2438-2443. (Biology). View Reference
  2. Hartley KO, Gell D, Smith GC. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product. Cell. 1995; 82(5):849-856. (Biology). View Reference
  3. Peterson SR, Stackhouse M, Waltman MJ, Chen F, Sato K, Chen DJ. Characterization of two DNA double-stranded break repair-deficient cell lines that express inactive DNA-dependent protein kinase catalytic subunits. J Biol Chem. 1997; 272(15):10227-10231. (Biology). View Reference
  4. Saitoh H, Pizzi MD, Wang J. Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358. J Biol Chem. 2002; 277(7):4755-4763. (Biology). View Reference
View All (4) View Less
610804 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.