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Purified Mouse Anti-HAP1
Purified Mouse Anti-HAP1

Western blot analysis of HAP1 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- HAP1 antibody.

Purified Mouse Anti-HAP1

Immunoflourescence staining of PC12 cells (rat neuroblastoma; ATCC CRL 1721).

Western blot analysis of HAP1 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- HAP1 antibody.

Immunoflourescence staining of PC12 cells (rat neuroblastoma; ATCC CRL 1721).

Product Details
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BD Transduction Laboratories™
Huntingtin Associated Protein 1
Rat (QC Testing), Mouse (Tested in Development)
Mouse IgG1
Rat HAP1-A aa. 100-289
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
85/98 kDa
250 µg/ml
AB_398828
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611302 Rev. 1
Antibody Details
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1/HAP1

Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by an expanding polyglutamine repeat in the IT15 or huntingtin gene. The mechanism(s) of pathogenesis are not known and the wide expression of Huntingtin protein does not explain the selective neuropathology of HD. HAP-1 (Huntingtin-associated protein 1), identified by yeast two hybrid screening, interacts with the huntingtin protein. There are two isoforms of rat HAP1 (HAP1-A and HAP1-B) which differ in the length of their C-terminal regions. Both proteins are highly hydrophilic and their binding to the Huntingtin protein is enhanced by the expanded polyglutamine repeat. Human HAP1 shares 62% amino acid identity with HAP1-A. HAP1 is specifically expressed in the CNS where it is restricted to limbic structures, such as amygdala, hypothalamus, bed nucleus of the stria terminalis, and the septal nucleus. The subcellular association of HAP1 with microtubules and many types of membraneous organelles implicates it in vesicular transport. Thus, the specific neural interaction of HAP1 with Huntingtin protein may lead to abnormalities in vesicular transport that cause the neuropathology of HD.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611302 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611302 Rev.1
Citations & References
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Development References (5)

  1. Gutekunst CA, Li SH, Yi H, Ferrante RJ, Li XJ, Hersch SM. The cellular and subcellular localization of huntingtin-associated protein 1 (HAP1): comparison with huntingtin in rat and human. J Neurosci. 1998; 18(19):7674-7686. (Biology). View Reference
  2. Li SH, Hosseini SH, Gutekunst CA, Hersch SM, Ferrante RJ, Li XJ. A human HAP1 homologue. Cloning, expression, and interaction with huntingtin. J Biol Chem. 1998; 273(30):19220-19227. (Biology). View Reference
  3. Li XJ, Li SH, Sharp AH, et al. A huntingtin-associated protein enriched in brain with implications for pathology. Nature. 1995; 378(6555):398-402. (Biology). View Reference
  4. Li XJ, Sharp AH, Li SH, Dawson TM, Snyder SH, Ross CA. Huntingtin-associated protein (HAP1): discrete neuronal localizations in the brain resemble those of neuronal nitric oxide synthase. Proc Natl Acad Sci U S A. 1996; 93(10):4839-4844. (Biology). View Reference
  5. Li Y, Chin LS, Levey AI, Li L. Huntingtin-associated protein 1 interacts with hepatocyte growth factor-regulated tyrosine kinase substrate and functions in endosomal trafficking. J Biol Chem. 2002; 277(31):28212-28221. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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611302 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.