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Purified Mouse Anti- Golgin-84
Purified Mouse Anti- Golgin-84

Western blot analysis of Golgin-84 on a SW-13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- Golgin-84 antibody.

Purified Mouse Anti- Golgin-84

Immunofluorescence staining of ES-2 cells (Human ovary clear cell carcinoma; ATCC CRL-1978).

Western blot analysis of Golgin-84 on a SW-13 cell lysate (Human adrenal gland carcinoma; ATCC CCL-105).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- Golgin-84 antibody.

Immunofluorescence staining of ES-2 cells (Human ovary clear cell carcinoma; ATCC CRL-1978).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Dog (Tested in Development)
Mouse IgG1
Human Golgin-84 aa. 510-713
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
84 kDa
250 µg/ml
AB_398905
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Antibody Details
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26/Golgin-84

The Golgi apparatus is a complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. Golgin-84 is an  integral membrane protein associated with the Golgi. Sequence analysis of the C-terminal region of Golgin-84 demonstrates a 14 residue region extending into the lumen of the Golgi, a membrane insertion sequence, and a Golgi retention signal. The large N-terminal cytoplasmic region contains a coiled-coil domain that is required for dimerization and two leucine zipper domains. Golgin-84 is ubiquitously expressed, but is abundant in testis. Golgin-84 has sequence homology to coiled-coil containing myosin family members and to several other Golgi proteins, including trans-Golgi p230 and gigantin. Trans-Golgi p230 has been implicated in the biogenesis of specific Golgi vesicles, while gigantin is implicated in the tethering of non-clathrin coated vesicles and in the anchoring of adjacent Golgi cisternae. Thus, Golgin-84 may have similar roles in vesicle biogenesis, docking, and fusion at the Golgi interface.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (1)

  1. Bascom RA, Srinivasan S, Nussbaum RL. Identification and characterization of golgin-84, a novel Golgi integral membrane protein with a cytoplasmic coiled-coil domain. J Biol Chem. 1999; 274(5):2953-2962. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.