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Purified Mouse Anti-DDB1
Purified Mouse Anti-DDB1

Western blot analysis of DDB1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-DDB1 antibody.

Purified Mouse Anti-DDB1

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Western blot analysis of DDB1 on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2.2).  Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-DDB1 antibody.

Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human DDB1 aa. 739-935
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
127 kDa
250 µg/ml
AB_399789
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612488 Rev. 1
Antibody Details
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8/DDB1

DNA lesions caused by chemical mutagens or radiation are corrected via nucleotide excision repair (NER). The NER system includes multiple proteins involved in xeroderma pigmentosum (XP) disorders, a pathology that causes hypersensitivity to sunlight and higher incidence of skin cancer. The proteins that cause these disorders are XP proteins and include XPA, XPB, XPC, XPD, XPF, and XPG. There are six repair complexes in the NER system composed of 15-18 proteins that include XPA, XPC, XPF, TFIIH, and hHR23B. In addition to these proteins, UV damage DNA-binding (UV-DDB) protein activity has also been associated with the NER system due to the fact that UV-DDB activity is absent in a subset of XPE Ddb- patients. UV-DDB consists of two subunits, DDB1 and DDB2, which can be injected into XPE cells to restore DNA repair synthesis. UV-DDB activity may be involved in the early stages of NER when it may promote recognition of the damaged DNA through DDB2. In addition, DDB1 can bind the histone acetyltransferase p300, which may be important for chromatin remodeling during the early stages of NER. Thus, UV-DDB activity may be important for recognition of specific types of DNA damage during NER.

612488 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612488 Rev.1
Citations & References
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Development References (4)

  1. Dualan R, Brody T, Keeney S, Nichols AF, Admon A, Linn S. Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein. Genomics. 1995; 29(1):62-69. (Biology). View Reference
  2. Fujiwara Y, Masutani C, Mizukoshi T, Kondo J, Hanaoka F, Iwai S. Characterization of DNA recognition by the human UV-damaged DNA-binding protein. J Biol Chem. 1999; 274(28):20027-20033. (Biology). View Reference
  3. Rapić-Otrin V, McLenigan MP, Bisi DC, Gonzalez M, Levine AS. Sequential binding of UV DNA damage binding factor and degradation of the p48 subunit as early events after UV irradiation. Nucleic Acids Res. 2002; 30(11):2588-2598. (Biology). View Reference
  4. Sun NK, Lu HP, Chao CC. Identification of rat DDB1, a putative DNA repair protein, and functional correlation with its damaged-DNA recognition activity. J Biomed Sci. 2002; 9(4):371-380. (Biology). View Reference
View All (4) View Less
612488 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.