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Purified Mouse Anti-CoRest
Purified Mouse Anti-CoRest

Western blot analysis of CoREST on a Jurkat lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- CoREST antibody.

Purified Mouse Anti-CoRest

Immunofluorescence staining of PSFK-1 cells, a human primitive neuroectodermal tumor line.

Western blot analysis of CoREST on a Jurkat lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- CoREST antibody.

Immunofluorescence staining of PSFK-1 cells, a human primitive neuroectodermal tumor line.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human CoREST aa. 78-192
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
66 kDa
250 µg/ml
AB_399517
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Antibody Details
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26/CoRest

Histone deacetylases (HDACs) are found in large, multisubunit complexes. HDAC1 and HDAC2 are found predominately in either an mSin3A-containing complex or a complex containing the ATP-dependent chromatin remodeling protein, CHD4. The mSin3A complex is recrutied by DNA-binding transcription factors, such as the unligand hormone receptors. In these situations, the HDAC/mSin3A complex is thought to be involved in gene repression. CoREST is a corepressor for REST (RE1 silencing transcription factor) that is found in complex with mSin3A, as well as in non-mSin3A HDAC complexes. CoREST contains an N-terminal and a C-terminal SANT domain, which is common to corepressors involved in steroid horomone receptor gene repression. In non-neuronal cells, CoREST and REST mediate repression of the type II sodium channel promoter. REST is usually found in complex with mSin3A, and under certain conditions may recruit CoREST for repressor activity. The N-terminal SANT domain of CoREST is required for interaction with HDAC1 to form an HDAC complex that does not contain mSin3A or REST. Thus, CoREST is a corepressor that may interact with distinct protein complexes depending on the target for gene repression.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

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Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
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Citations & References
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Development References (3)

  1. Andres ME, Burger C, Peral-Rubio MJ, et al. CoREST: a functional corepressor required for regulation of neural-specific gene expression. Proc Natl Acad Sci U S A. 1999; 96(17):9873-9878. (Biology). View Reference
  2. Grimes JA, Nielsen SJ, Battaglioli E, et al. The co-repressor mSin3A is a functional component of the REST-CoREST repressor complex. J Biol Chem. 2000; 275(13):9461-9467. (Biology). View Reference
  3. You A, Tong JK, Grozinger CM, Schreiber SL. CoREST is an integral component of the CoREST- human histone deacetylase complex. Proc Natl Acad Sci U S A. 2001; 98(4):1454-1458. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.