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Purified Mouse Anti-CD49c
Purified Mouse Anti-CD49c

Western blot analysis of CD49c (integrin α3) on a rat kidney lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-CD49c antibody.

Purified Mouse Anti-CD49c

Immunofluorescence staining of MDCK cells (canine kidney; ATCC CCL-34).

Western blot analysis of CD49c (integrin α3) on a rat kidney lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-CD49c antibody.

Immunofluorescence staining of MDCK cells (canine kidney; ATCC CCL-34).

Product Details
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BD Transduction Laboratories™
Integrin α3; VLA-3α
Rat (QC Testing), Mouse, Dog (Tested in Development)
Mouse IgG1
Mouse VLA-3α aa. 110-325
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
135 kDa
250 µg/ml
AB_398358
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611045 Rev. 1
Antibody Details
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42/CD49c

Integrins are a family of dimeric proteins that mediate cell-to-cell and extracellular matrix adhesion.  They consist of a large α chain that is non-covalently associated with a smaller β chain which defines the integrin subfamilies.  VLA-3 (Very Late Antigen-3), a member of the integrin superfamily, exhibits elevated expression on B lymphocytes, but is also found on monocytes, platelets, and hematopoietic progenitor cells.  A heterodimer of α3 (CD49c) and β1 (CD29) subunits, VLA is a receptor for laminin, fibronectin, and collagen.  The α3 chain contains a large extracellular domain with three putative metal-binding sequences, a transmembrane domain, and a short cytoplasmic tail.  Differing requirements for divalent cations and the influence of RGD peptides results in multiple ligand-binding mechanisms for VLA-3.  Although its expression is restricted in normal tissues, VLA-3 is found on a variety of cultured tumor cells.  In addition, levels of VLA-3 have been shown to correlate with the degree of invasiveness of malignant melanoma cells.  Thus, VLA-3 mediates intercellular adhesion and cell migration in normal and, possibly, cancerous cell types.

611045 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611045 Rev.1
Citations & References
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Development References (2)

  1. Takada Y, Murphy E, Pil P, Chen C, Ginsberg MH, Hemler ME. Molecular cloning and expression of the cDNA for alpha 3 subunit of human alpha 3 beta 1 (VLA-3), an integrin receptor for fibronectin, laminin, and collagen. J Cell Physiol. 1991; 115(1):257-266. (Biology). View Reference
  2. Takeuchi K, Hirano K, Tsuji T, Osawa T, Irimura T. cDNA cloning of mouse VLA-3 alpha subunit. J Cell Biochem. 1995; 57(2):371-377. (Biology). View Reference
611045 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.