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Purified Mouse Anti-basic FGF
Purified Mouse Anti-basic FGF

Western blot analysis for basic FGF on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti-basic FGF antibody.

Purified Mouse Anti-basic FGF

Immnuofluroescence staining of human fibroblasts.

Western blot analysis for basic FGF on a HeLa cell lysate (Human cervical epitheloid carcinoma; ATCC CCL-2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti-basic FGF antibody.

Immnuofluroescence staining of human fibroblasts.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG2a
Human bFGF aa. 1-155
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
18-24 kDa
250 µg/ml
AB_399693
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
610073 Rev. 1
Antibody Details
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6/basic FGF

Basic Fibroblast Growth Factor (bFGF) is a family member of the cell-differentiating and growth promoting factors. Basic FGF exists as multiple isoforms ranging from 18-24kDa that are derived from a single mRNA species under unique translational conditions. The 18kDa isoform is primarily cytosolic, but is also secreted and may form reservoirs of bFGF in the extracellular matrix. In contrast, the larger isoforms are predominantly nuclear. At the cellular level, bFGF is a potent mitogen and promotes cell survival by inhibiting apoptosis. At the tissue level, it is involved in wound repair and induces angiogenesis. In addition, bFGF is a significant target in cancer research because it is over-expressed in some cancers and may enhance a tumor's metastatic potential.

610073 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610073 Rev.1
Citations & References
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Development References (5)

  1. Estival A, Monzat V, Miquel K. Differential regulation of fibroblast growth factor (FGF) receptor-1 mRNA and protein by two molecular forms of basic FGF. Modulation of FGFR-1 mRNA stability. J Biol Chem. 1996; 271(10):5663-5670. (Biology). View Reference
  2. Fox JC, Shanley JR. Antisense inhibition of basic fibroblast growth factor induces apoptosis in vascular smooth muscle cells. J Biol Chem. 1996; 271(21):12578-12584. (Biology). View Reference
  3. Peng H, Moffett J, Myers J. Novel nuclear signaling pathway mediates activation of fibroblast growth factor-2 gene by type 1 and type 2 angiotensin II receptors. Mol Cell Biol. 2001; 12(2):449-462. (Biology: Western blot). View Reference
  4. Stachowiak MK, Moffett J, Joy A. Regulation of bFGF gene expression and subcellular distribution of bFGF protein in adrenal medullary cells. J Cell Biol. 1994; 127(1):203-223. (Biology). View Reference
  5. Yaccoby S, Johnson CL, Mahaffey SC, Wezeman MJ, Barlogie B, Epstein J. Antimyeloma efficacy of thalidomide in the SCID-hu model. Blood. 2002; 100(12):4162-4168. (Biology: Immunohistochemistry). View Reference
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610073 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.