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Caveolin Sampler Kit

Caveolin Sampler Kit

(RUO)
Caveolin Sampler Kit

IP: nat = native condition, den = denaturing conditions

This kit includes 10 µg of each antibody listed at a concentration of 250 µg/ml. No substitutions allowed.

IP: nat = native condition, den = denaturing conditions

This kit includes 10 µg of each antibody listed at a concentration of 250 µg/ml. No substitutions allowed.

Product Details
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BD Transduction Laboratories™
250 µg/ml
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
RUO
AB_2870072


Description

Identified as a tyrosine phosphorylated protein in Rous sarcoma virus-transformed chick embryo fibroblasts (CEF), caveolin is now known to be ubiquitously expressed. Caveolin (also known as VIP21) localizes to non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. This transmembrane protein plays a structural role in these specializations. Caveolin is also present at the trans-Golgi network (TGN) and similar quantities are found in apically and basolaterally destined transport vesicles. Caveolin-1 is present in two forms, α and β, due to alternate splicing of the mRNA. The α isoform has been reported to be observed at 24 kD and the β isoform at 21 kD.  During caveolae formation, caveolin-1 forms large lipid-binding homo-oligomers. In addition, phosphorylation of caveolin at Tyr-14, Ser-88, and other residues in v-src transformed cells leads to flattening, aggregation, and fusion of caveolae and caveolae-derived vesicles. Caveolin-1 may also function as a scaffolding protein, which organizes signaling molecules. This functional role is supported by the fact that caveolin-1 interacts directly with inactive ras and G-protein α subunits. Although caveolin 2 is similar to caveolin 1 in distribution and tissue expression, caveolin 2 is most abundant in adipose tissue and its expression is up-regulated upon differentiation. Caveolin 3 has been identified as a distinct isoform which is expressed only in smooth, skeletal, and cardiac muscle. Flotillin-1 was also isolated from the Triton X-100 insoluble buoyant fraction that includes caveolae. Although the mRNA expression of both flotillin-1 and Caveolin is very similar, caveolin is undetectable in brain, while flotillin-1 is very abundant. Flotillin-1 is a close homolog of the Epidermal Surface Antigen (ESA/flotillin-2), which also colocalizes in the caveolae. Thus, flotillin-1 and its relative ESA/flotillin-2 are also incorporated into the expanding list of proteins co-localized with caveolae, which includes PKCα, Ras, Rap Src-like kinases, Gαβγ, GPI-linked receptors and Nitric Oxide Synthases.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611766 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.