Skip to main content Skip to navigation
Purified Mouse Anti-Human IL-9

Purified Mouse Anti-Human IL-9

Clone MH9A4

(RUO)
Purified Mouse Anti-Human IL-9

Curve from a sandwich ELISA that measures IL-9 protein levels.  It was generated using the purified MH9A4 antibody as capture antibody, doubling dilutions of recombinant human IL-9 protein as standard and biotinylated MH9A3 antibody as detection antibody.  Avidin-HRP and the TMB substrate were used to develop the detection stage and mean OD was measured at 450 - 570 nm.  The standard curve is displayed as the concentration of recombinant human IL-9 in pg/ml versus the microwell absorbance (in circles).

Curve from a sandwich ELISA that measures IL-9 protein levels.  It was generated using the purified MH9A4 antibody as capture antibody, doubling dilutions of recombinant human IL-9 protein as standard and biotinylated MH9A3 antibody as detection antibody.  Avidin-HRP and the TMB substrate were used to develop the detection stage and mean OD was measured at 450 - 570 nm.  The standard curve is displayed as the concentration of recombinant human IL-9 in pg/ml versus the microwell absorbance (in circles).

Product Details
Down Arrow Up Arrow


BD Pharmingen™
0.5 mg/ml
Human (QC Testing)
ELISA Capture (Routinely Tested)
RUO
AB_647279
Aqueous buffered solution containing ≤0.09% sodium azide.


Description

The MH9A4 monoclonal antibody reacts with human interleukin-9 (IL-9), 14.1 kDa T cell-derived multifunctional cytokine . IL-9 induces the proliferation and differentiation of various lymphoid (eg, T lymphocytes, B lymphocytes, mast cells, eosinophils, and neutrophils) and hemopoietic cells,  It potentiates the interleukin-4-induced IgG and IgE responses by human B lymphocytes. Further, IL-9 has been implicated in human allergic disorders.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

This  antibody is useful as a capture antibody in ELISA assays measuring human IL-9 protein levels.  This antibody is non-blocking, it does not block the binding of IL-9 with the IL-9 receptor.  The MH9A4 mAb can be used as a capture antibody in a sandwich ELISA with Biotinylated MH9A3 anti-human IL-9 protein (Cat. No. 558089) as the detector antibody, with recombinant human IL-9 protein as the standard.  The MH9A4 antibody should be titrated between 1-4 µg/ml to determine its optimal ELISA coating concentration.  To obtain linear standard curves, doubling dilutions of the recombinant human IL-9 protein ranging from 1.0 to 125 pg/ml are recommended for inclusion in each ELISA plate.  For specific methodology, see Chapter 7: ELISA for specifically measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors. 2003. Techniques for Immune Function Analysis application Handbook 1st Edition. BD Biosciences.  Available for download as the Immune Function Application handbook from http://www.bdbiosciences.com/cgi-bin/literature/archive?type=2&brand=0.

Note: This ELISA antibody pair shows no crossreactivity with the following recombinant human cytokines: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL5, IL-6, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-18, IFNγ, gp130, TNF, 4-1BB. This ELISA antibody pair also shows no cross-reactivity with recombinant mouse IL-9.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558088 Rev. 2
Citations & References
Down Arrow Up Arrow

Development References (5)

  1. Demoulin JB, Renauld JC. Interleukin 9 and its receptor: an overview of structure and function. Int Rev Immunol. 1998; 16(3-4):345-364. (Biology). View Reference
  2. Dugas B, Renauld JC, Pène J, et al. Interleukin-9 potentiates the interleukin-4-induced immunoglobulin (IgG, IgM and IgE) production by normal human B lymphocytes. Eur J Immunol. 1993 July; 23(7):1687-1692. (Biology). View Reference
  3. Jenmalm MC, Van Snick J, Cormont F, Salman B. Allergen-induced Th1 and Th2 cytokine secretion in relation to specific allergen sensitization and atopic symptoms in children. Clin Exp Allergy. 2001 October; 31(10):1528-1535. (Biology). View Reference
  4. Renauld JC. IL-9. In: Oppenheim JJ, Feldmann M, Durum SK. editors in chief, Joost J. Oppenheim, Marc Feldman ; editors, Scott K. Durum .. et al., ed. Cytokine reference : a compendium of cytokines and other mediators of host defense. London: Academic Press; 2001:155-164.
  5. Renauld JC. New insights into the role of cytokines in asthma. J Clin Pathol. 2001 August; 54(8):577-589. (Biology). View Reference
558088 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.