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Purified Mouse Anti-Human CD154
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This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
ELISA Capture (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

ELISA Capture: Purified Mouse Anti-Human CD154 antibody (Cat. No. 552559) is useful as a capture antibody for a sandwich ELISA for measuring human CD40L protein levels. Purified Mouse Anti-Human CD154 antibody can be paired with Biotin Mouse Anti-Human CD154 (Cat. No. 552560) as the detecting antibody.  Purified Mouse Anti-Human CD154 antibody should be titrated 1 - 4 µg/ml to determine the optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant human soluble CD40L, ranging from 2000 to 15 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology see the "ELISA and ELISPOT" section at our website:

Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human proteins: CD40, IL-1Ra, IL-1R1, IL-1RII, IL-1α, IL-1β, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF-α, TNF-β, sTNFRI, sTNFRII, IFNγ, TRAIL, MCSF, TGFβ, Trx

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Please refer to to access safety data sheets (SDS).
  5. Please refer to for technical protocols.
552559 Rev. 3
Antibody Details
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Clone hCD40L-M90.1 reacts with CD40 ligand (CD40L), a 39 kDa type II membrane glycoprotein expressed on activated T cells. CD40-CD40L interaction plays a very important role in T cell-dependent B-cell proliferation, differentiation, and memory cell formation. In addition, blocking of CD40-CD40L interaction has been demonstrated with soluble CD40, resulting in the inhibition of immunoglobulin isotype switching. It has been reported that patients with X-linked hyper-IgM syndrome have defective expression of functional CD40L due to a defective gene that encodes CD40L.

552559 Rev. 3
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
552559 Rev.3
Citations & References
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Development References (4)

  1. Fuleihan R, Ramesh N, Horner A, et al. Cyclosporin A inhibits CD40 ligand expression in T lymphocytes. J Clin Invest. 1994; 93(3):1315-1320. (Biology). View Reference
  2. Gray D, Dullforce P, Jainandunsing S. Memory B cell development but not germinal center formation is impaired by in vivo blockade of CD40-CD40 ligand interaction. J Exp Med. 1994; 180(1):141-155. (Biology). View Reference
  3. Nishioka Y, Lipsky PE. The role of CD40-CD40 ligand interaction in human T cell-B cell collaboration. J Immunol. 1994; 153(3):1027-1036. (Biology). View Reference
  4. van Kooten C, Gaillard C, Galizzi JP, et al. B cells regulate expression of CD40 ligand on activated T cells by lowering the mRNA level and through the release of soluble CD40. Eur J Immunol. 1994; 24(4):787-792. (Biology). View Reference
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552559 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.