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Purified Mouse Anti-Glutathione S-Transferase
Product Details
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BD BaculoGold™
GST
Mouse IgG1
Recombinant GST-tagged protein
Western blot (Routinely Tested), ELISA (Tested During Development)
26 kDa
0.5 mg/ml
AB_395536
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Applications include western blot analysis (1-2 µg/ml antibody). The antibody is useful in screening for recombinant GST-tagged proteins produced in bacteria or in insect cells infrected with recombinant baculovirus.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554805 Rev. 6
Antibody Details
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G172-1138

Glutathione S-Transferase (GST) from Schistosoma japonicum is a 26 kD protein that has become a popular fusion partner for high expression of recombinant proteins. GST tags allow recombinant fusion proteins to be purified in a single nondenaturing affinity step on glutathione-coupled beads. The high affinity of the GST-glutathione interaction results in simple near-homogeneous purifications. Clone G172-1138 recognizes GST. A recombinant GST-tagged protein was used as immunogen.

554805 Rev. 6
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
554805 Rev.6
Citations & References
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Development References (1)

  1. Davies AH, Jowett JB, Jones IM. Recombinant baculovirus vectors expressing glutathione-S-transferase fusion proteins. Biotechnology. 1993; 11(8):933-936. (Biology). View Reference
554805 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.