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RB744 Mouse Anti-Integrin α7 (ITGA7)
Product Details
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BD OptiBuild™
ITGA7; Integrin α7; integrin alpha-7; α7 Integrin
Human,Mouse (Tested in Development)
Mouse C57BL/6 IgG1, κ
Mouse Myoblasts
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  4. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
  7. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  10. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  11. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  12. For U.S. patents that may apply, see bd.com/patents.
757804 Rev. 1
Antibody Details
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3C12

The 3C12 monoclonal antibody specifically recognizes Integrin alpha 7 (Integrin α7 or α7 Integrin). Integrins mediate important intercellular and cell-extracellular matrix interactions in a variety of biological processes. Integrin α7 is a single-pass type I transmembrane glycoprotein that is encoded by ITGA7 (Integrin subunit alpha 7) which belongs to the Integrin alpha chain family. Integrin α7 is mainly expressed by skeletal, cardiac, and smooth muscle cells. It associates with Integrin β1 to form a heterodimeric Integrin α7β1 complex. This heterodimer functions as the major laminin-binding receptor. Integrin α7β1 mediates cellular binding to components of the extracellular matrix including laminin-1 as well as the laminin isoforms-2 and -4. Integrin α7β1 participates in the adhesion and motility of myoblasts during myogenesis and helps guide muscle fiber formation and maintenance.

757804 Rev. 1
Format Details
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RB744
The BD Horizon RealBlue™ 744 (RB744) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 746-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB744 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), we recommend using an optical filter centered near 750-nm (e.g., a 750/60-nm bandpass filter).
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RB744
Blue 488 nm
498 nm
746 nm
757804 Rev.1
Citations & References
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View product citations for antibody "757804" on CiteAb

Development References (10)

  1. Burkin DJ, Kaufman SJ. The alpha7beta1 integrin in muscle development and disease.. Cell Tissue Res. 1999; 296(1):183-90. (Biology). View Reference
  2. Kobayashi N, Oda T, Takizawa M, et al. Integrin α7 and Extracellular Matrix Laminin 211 Interaction Promotes Proliferation of Acute Myeloid Leukemia Cells and Is Associated with Granulocytic Sarcoma.. Cancers (Basel). 2020; 12(2):E363. (Biology). View Reference
  3. Liu J, Burkin DJ, Kaufman SJ. Increasing alpha 7 beta 1-integrin promotes muscle cell proliferation, adhesion, and resistance to apoptosis without changing gene expression.. Am J Physiol Cell Physiol. 2008; 294(2):C627-40. (Biology). View Reference
  4. Mayer U, Saher G, Fässler R, et al. Absence of integrin alpha 7 causes a novel form of muscular dystrophy.. Nat Genet. 1997; 17(3):318-23. (Biology). View Reference
  5. Mielenz D, Hapke S, Pöschl E, von Der Mark H, von Der Mark K. The integrin alpha 7 cytoplasmic domain regulates cell migration, lamellipodia formation, and p130CAS/Crk coupling.. J Biol Chem. 2001; 276(16):13417-26. (Biology: Flow cytometry). View Reference
  6. Rosbottom A, Scudamore CL, von der Mark H, Thornton EM, Wright SH, Miller HR. TGF-beta 1 regulates adhesion of mucosal mast cell homologues to laminin-1 through expression of integrin alpha 7.. J Immunol. 2002; 169(10):5689-95. (Clone-specific: Flow cytometry, Functional assay). View Reference
  7. Schöber S, Mielenz D, Echtermeyer F, et al. The role of extracellular and cytoplasmic splice domains of alpha7-integrin in cell adhesion and migration on laminins.. Exp Cell Res. 2000; 255(2):303-13. (Immunogen). View Reference
  8. Su Y, Guan XQ, Liu FQ, Wang YL. The effects of MIBG on the invasive properties of HepG2 hepatocellular carcinoma cells.. Int J Mol Med. 2014; 34(3):842-8. (Biology). View Reference
  9. Ziober BL, Vu MP, Waleh N, Crawford J, Lin CS, Kramer RH. Alternative extracellular and cytoplasmic domains of the integrin alpha 7 subunit are differentially expressed during development.. J Biol Chem. 1993; 268(35):26773-83. (Biology). View Reference
  10. von der Mark H, Williams I, Wendler O, Sorokin L, von der Mark K, Pöschl E. Alternative splice variants of alpha 7 beta 1 integrin selectively recognize different laminin isoforms.. J Biol Chem. 2002; 277(8):6012-6. (Clone-specific: Functional assay). View Reference
View All (10) View Less
757804 Rev. 1

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.