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Purified Mouse Anti-Human CD153
Product Details
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BD Pharmingen™
CD30L, CD30 ligand
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
550052 Rev. 6
Antibody Details
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The D2-1173 monoclonal antibody specifically binds to the CD30 ligand (CD153) a 40 kDa type II membrane glycoprotein expressed mainly on activated T cells, as well as on activated macrophages, neutrophils and both normal and malignant B cells. CD30 ligand is a member of the tumor necrosis factor (TNF) superfamily. In addition to TNF, this family also includes lymphotoxin α (LTα), LTβ, CD154, CD95L, CD70, CD134 ligand, CDw137 ligand, and TRAIL. Results of in vitro studies have shown that CD30 mediates a variety of activation and differentiation, or apoptotic signals. Recently, it has been reported that CD30-deficient mice have elevated numbers of  thymocytes and show a gross defect in negative but not positive selection during T cell differentiation, suggesting an important role for CD30/CD30L interactions in the deletion of autoreactive T cells.

550052 Rev. 6
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
550052 Rev.6
Citations & References
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Development References (5)

  1. Del Prete G, De Carli M, D'Elios MM. CD30-mediated signaling promotes the development of human T helper type 2-like T cells. J Exp Med. 1995; 182(6):1655-1661. (Biology). View Reference
  2. Gruss HJ, Pinto A, Gloghini A. CD30 ligand expression in nonmalignant and Hodgkin's disease-involved lymphoid tissues. Am J Pathol. 1996; 149(2):469-481. (Biology). View Reference
  3. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
  4. Romagnani P, Annunziato F, Manetti R. High CD30 ligand expression by epithelial cells and Hassal's corpuscles in the medulla of human thymus. Blood. 1998; 91(9):3323-3332. (Biology). View Reference
  5. Smith CA, Gruss HJ, Davis T. CD30 antigen, a marker for Hodgkin's lymphoma, is a receptor whose ligand defines an emerging family of cytokines with homology to TNF. Cell. 1993; 73(7):1349-1360. (Biology). View Reference
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550052 Rev. 6

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.