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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
The 2F3 monoclonal antibody specifically recognizes CD369 which is also known as Dectin-1 (Dendritic cell-associated C-type lectin 1) or BGR (Beta-glucan receptor). CD369 is a ~ 43 kDa type II transmembrane C-type lectin that is encoded by Clec7a (C-type lectin domain family 7, member a). The extracellular portion of CD369 (Clec7a) contains a C-terminal stalk with a carbohydrate recognition domain (CRD) that is followed by a transmembrane segment, and an ITAM-containing cytoplasmic tail. This protein is predominantly expressed on monocytes/macrophages, neutrophils and some dendritic cell populations and at a lower level on a sub-population of T cells. CD369 (Clec7a) binds to beta-glucan polymers and functions as a pattern recognition receptor (PRR) in innate immune response to fungal and bacterial pathogens. CD369 mediated signaling may play a role in leucocyte responses, including phagocytosis or enhanced cytokine production. In mice, two functionally different full-length and stalkless isoforms have been characterized. Both isoforms vary in their ability to recognize zymosan and mediate cellular responses upon zymosan recognition.
Development References (4)
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Brown GD, Taylor PR, Reid DM, et al. Dectin-1 is a major beta-glucan receptor on macrophages.. J Exp Med. 2002; 196(3):407-12. (Biology). View Reference
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Herre J, Gordon S, Brown GD. Dectin-1 and its role in the recognition of beta-glucans by macrophages.. Mol Immunol. 2004; 40(12):869-76. (Biology). View Reference
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Taylor PR, Brown GD, Reid DM, et al. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages.. J Immunol. 2002; 169(7):3876-82. (Biology). View Reference
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Underhill DM, Rossnagle E, Lowell CA, Simmons RM. Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production.. Blood. 2005; 106(7):2543-50. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.