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      PE Mouse Anti-Mouse CD182 (CXCR2)
      PE Mouse Anti-Mouse CD182 (CXCR2)
      Multicolor flow cytometric analysis of CD182 (CXCR2) expression on C57BL/6 and BALB/c mouse myeloid cells. Bone marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse Ly-6C and Ly-6G (Gr-1) antibody (Cat. No. 553129/561083) and with either BD PE Mouse IgG2a, κ Isotype Control (Cat. No. 553457; Left Plots) or PE Mouse Anti-Mouse CD182 (CXCR2) antibody (Cat. No. 567848; Right Plots) at 0.5 µg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD182 (CXCR2) [or Ig Isotype control staining] versus Ly-6C and Ly-6G, were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      PE Mouse Anti-Mouse CD182 (CXCR2)
      Multicolor flow cytometric analysis of CD182 (CXCR2) expression on C57BL/6 and BALB/c mouse myeloid cells. Bone marrow cells from BALB/c mice were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with APC Rat Anti-Mouse Ly-6C and Ly-6G (Gr-1) antibody (Cat. No. 553129/561083) and with either BD PE Mouse IgG2a, κ Isotype Control (Cat. No. 553457; Left Plots) or PE Mouse Anti-Mouse CD182 (CXCR2) antibody (Cat. No. 567848; Right Plots) at 0.5 µg/test. DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The pseudocolor density plots showing the correlated expression of CD182 (CXCR2) [or Ig Isotype control staining] versus Ly-6C and Ly-6G, were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) bone marrow leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      Cxcr2; CXC-R2; CXCR-2;Cmkar2; IL-8R B; IL-8rb; Il8rb
      Mouse (QC Testing)
      Mouse C57BL/6 IgG2c, κ
      Mouse CXCR2-transfected Cells
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      6. An isotype control should be used at the same concentration as the antibody of interest.
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      567848 Rev. 3
      Antibody Details
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      3F10_B3

      Clone 3F10_B3 is a mouse monoclonal antibody (also known as, clone 3F10) that specifically recognizes mouse CD182 which is also known as C-X-C chemokine receptor type 2 (CXCR2) or Interleukin-8 Receptor B (IL-8RB). The  CD182 (CXCR2) chemokine receptor is a seven-transmembrane protein encoded by Cxcr2 [Chemokine (C-X-C motif) receptor 2] which belongs to the G protein-coupled receptor 1 family. CD182 (CXCR2) is highly expressed on neutrophils and binds to several chemokines including CXCL1 (GROα/KC), CXCL2 (GROβ/MIP2), CXCL3 (MIP2β/DCIP-1), CXCL5 (LIX), and CXCL7 (NAP-2). CD182 (CXCR2) plays a role in neutrophil activation and trafficking. The 3F10_B3 antibody was raised against Mouse CXCR2-transfected cells.

      567848 Rev. 3
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      567848 Rev.3
      Citations & References
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      View product citations for antibody "567848" on CiteAb

      Development References (3)

      1. Bachelerie F, Ben-Baruch A, Burkhardt AM, et al. International Union of Basic and Clinical Pharmacology. [corrected]. LXXXIX. Update on the extended family of chemokine receptors and introducing a new nomenclature for atypical chemokine receptors.. Pharmacol Rev. 2014; 66(1):1-79. (Biology). View Reference
      2. Bozic CR, Gerard NP, von Uexkull-Guldenband C, et al. The murine interleukin 8 type B receptor homologue and its ligands. Expression and biological characterization.. J Biol Chem. 1994; 269(47):29355-8. (Biology). View Reference
      3. Griffith JW, Sokol CL, Luster AD. Chemokines and chemokine receptors: positioning cells for host defense and immunity.. Annu Rev Immunol. 2014; 32:659-702. (Biology). View Reference
      567848 Rev. 3

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.