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PE Mouse Anti-Human CD112
Product Details
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BD Pharmingen™
HVEB; PRR2; PVRL2; PVRR2; NECTIN2; nectin-2; nectin 2
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
20 µl
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to for technical protocols.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
551057 Rev. 2
Antibody Details
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The R2.525 monoclonal antibody specifically binds to CD112. CD112 is a type I transmembrane glycoprotein that is encoded by NECTIN2 (Nectin cell adhesion molecule 2) which belongs to the Ig gene superfamily. CD112 is also known as polio virus related receptor 2 (PRR2), poliovirus receptor-like 2 (PVRL2) or herpesvirus entry protein B (HveB). CD112 is a cell surface molecule homologous to the poliovirus receptor (PVR/CD155). The NECTIN2 gene codes for two mRNA isoforms, a short form of 3.0 kb (hPRR2α) and a long form of 4.4 kb (hPRR2δ). Both are widely expressed in human tissues, including hematopoietic cells. CD112 is also expressed on endothelial cells, at the intercellular junctions of adjacent cells. PRR2 may play a role in the regulation of hematopoietic/endothelial cell interactions.

551057 Rev. 2
Format Details
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
551057 Rev.2
Citations & References
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Development References (2)

  1. Eberle F, Dubreuil P, Mattei MG, Devilard E, Lopez M. The human PRR2 gene, related to the human poliovirus receptor gene (PVR), is the true homolog of the murine MPH gene. Gene. 1995; 159(2):267-272. (Biology). View Reference
  2. Lopez M, Aoubala M, Jordier F, Isnardon D, Gomez S, Dubreuil P. The human poliovirus receptor related 2 protein is a new hematopoietic/endothelial homophilic adhesion molecule. Blood. 1998; 92(12):4602-4611. (Biology). View Reference
551057 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.