Stage-specific embryonic antigen (SSEA)-5 is a pluripotency surface marker expressed in the blastocyst inner cell mass and on human pluripotent stem cells, including both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Because SSEA-5 expression rapidly decreases upon differentiation, it can be used to identify undifferentiated pluripotent stem cells. SSEA-5 can be combined with other pluripotency surface markers (e.g. CD9/CD90 or CD50/CD200) to immunodeplete remaining pluripotent stem cells from incompletely differentiated cultures.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.