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BUV737 Rat Anti-Mouse CD26
Product Details
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BD OptiBuild™
Cd26; Dpp4; dipeptidylpeptidase 4; THAM, DPP IV
Mouse (Tested in Development)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Cell-surface molecules solubilized from BALB/c mouse thymocytes
Flow cytometry (Qualified)
0.2 mg/ml
AB_2871099
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV737 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 737 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
  10. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
741729 Rev. 2
Antibody Details
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H194-112

The H194-112 monoclonal antibody specifically binds to CD26, which is also known as, Thymocyte-activating molecule (THAM), or dipeptidyl peptidase IV (DPP IV, Dpp4).  CD26 is a ~220- kDa dimer formed of identical type-II transmembrane core polypeptides which undergo variable post-translational modifications.  It is a multi-functional molecule with both ectopeptidase and signal-transducing activities.  Studies with specific DPP IV inhibitors suggest that the enzymatic activity is involved in the mediation of T-cell activation events.  The expression of CD26 is developmentally regulated in the thymus.  Resting lymphoid cells of the bone marrow and peripheral B and T lymphocytes express low levels of CD26; bone-marrow and peritoneal myeloid cells do not.  CD26 is also found on epithelial cells in the kidney, liver, small intestine, and lung.  Cross-linked H194-112 mAb induces proliferation of immature and mature thymocytes in the presence of either IL-1 plus IL-2 or PMA;  addition of IL-2 or IL-4 to PMA further enhances the activation.

The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter.  Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor® 700-like dyes (eg, 712/20-nm filter).

Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

741729 Rev. 2
Format Details
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BUV737
The BD Horizon Brilliant™ Ultraviolet 737 (BUV737) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 735-nm. BUV737, driven by BD innovation, is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 740-nm (e.g., 740/35 bandpass filter). The acceptor dye can be excited by the Red (628–640nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BUV737
Ultraviolet 355 nm
350 nm
735 nm
741729 Rev.2
Citations & References
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Development References (7)

  1. Fleischer B. CD26: a surface protease involved in T-cell activation. Immunol Today. 1994; 15(4):180-184. (Biology). View Reference
  2. Gorvel JP, Vivier I, Naquet P, Brekelmans P, Rigal A, Pierres M. Characterization of the neutral aminopeptidase activity associated to the mouse thymocyte-activating molecule. J Immunol. 1990; 144(8):2899-2907. (Biology). View Reference
  3. Marguet D, Bernard AM, Vivier I, Darmoul D, Naquet P, Pierres M. cDNA cloning for mouse thymocyte-activating molecule. A multifunctional ecto-dipeptidyl peptidase IV (CD26) included in a subgroup of serine proteases. J Biol Chem. 1992; 267(4):2200-2208. (Biology). View Reference
  4. Naquet P, MacDonald HR, Brekelmans P. A novel T cell-activating molecule (THAM) highly expressed on CD4-CD8- murine thymocytes. J Immunol. 1988; 141(12):4101-4109. (Immunogen: Activation). View Reference
  5. Naquet P, Vivier I, Gorvel JP. Activation of mouse T lymphocytes by a monoclonal antibody to a developmentally regulated surface aminopeptidase (THAM). Immunol Rev. 1989; 111:177-193. (Clone-specific: Activation). View Reference
  6. Reinhold D, Bank U, Buhling F. Inhibitors of dipeptidyl peptidase IV (DP IV, CD26) induces secretion of transforming growth factor-beta 1 (TGF-beta 1) in stimulated mouse splenocytes and thymocytes. Immunol Lett. 1997; 58(1):29-35. (Biology: Activation). View Reference
  7. Vivier I, Marguet D, Naquet P . Evidence that thymocyte-activating molecule is mouse CD26 (dipeptidyl peptidase IV). J Immunol. 1988; 147(2):447-454. (Biology). View Reference
View All (7) View Less
741729 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.