Skip to main content Skip to navigation
BUV661 Mouse Anti-Human CD220
Product Details
Down Arrow Up Arrow

BD OptiBuild™
Insulin Receptor; Insulin R; IR; INSR; HHF5
Human (Tested in Development)
Mouse IgG1, κ
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV661 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  7. Please refer to for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Ultraviolet 661 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,575,303; 8,354,239.
750653 Rev. 4
Antibody Details
Down Arrow Up Arrow

The 3B6 monoclonal antibody specifically recognizes the Insulin Receptor (IR), a glycoprotein composed of two extracellular α-subunits (130 kDa) and two transmembrane β-subunits (95 kDa). It is expressed on human hematopoietic and non-hematopoietic cells, but unlike the insulin-like growth factor receptor (IGF-IR), which is ubiquitous, the insulin receptor is restricted to major target tissues of insulin action. Upon binding insulin, the IR subunits form a heterotetramer of two α and two β subunits resulting in autophosphorylation and activation of the tyrosine kinase activity of the receptor. This ligand-receptor interaction is important in regulating cell metabolism and growth. 3B6/IR monoclonal antibody reacts similarly to anti-human IR α, clone 47-9, an α-subunit antibody.

The antibody was conjugated to BD Horizon™ BUV661 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 661-nm. BD Horizon Brilliant BUV661 can be excited by the ultraviolet laser (355 nm) and detected with a 670/25 filter and a 630 nm LP.  Due to cross laser excitation of this dye, there may be significant spillover into channels detecting APC-like emissions (eg, 670/25-nm filter).


Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV661 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV661 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone-specific compensation controls when using these reagents.

750653 Rev. 4
Format Details
Down Arrow Up Arrow
The BD Horizon Brilliant™ Ultraviolet 661 (BUV661) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 660-nm. BUV661, driven by BD innovation, is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 660-nm (e.g., 670/25 bandpass filter). The acceptor dye can be excited by the Red (628–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Ultraviolet 355 nm
350 nm
660 nm
750653 Rev.4
Citations & References
Down Arrow Up Arrow

Development References (7)

  1. Brindle NP, Tavare JM, Dickens M, Whittaker J, Siddle K. Anti-(insulin receptor) monoclonal antibody-stimulated tyrosine phosphorylation in cells transfected with human insulin receptor cDNA. Biochem J. 1990; 268(3):615-620. (Biology). View Reference
  2. Hagino H, Shii K, Yokono K, et al. Enzyme-linked immunosorbent assay method for human autophosphorylated insulin receptor. Applicability to insulin-resistant states.. Diabetes. 1994; 43(2):274-80. (Clone-specific: ELISA). View Reference
  3. Okada Y, Yoshida M, Baba S, Shii K. Development of vanadate sensitive human erythrocytes insulin receptor tyrosine phosphatase assay.. Diabetes Res Clin Pract. 1998; 41(3):157-63. (Clone-specific). View Reference
  4. Prigent SA, Stanley KK, Siddle K. Identification of epitopes on the human insulin receptor reacting with rabbit polyclonal antisera and mouse monoclonal antibodies. J Biol Chem. 1990; 265(17):9970-9979. (Biology). View Reference
  5. Shima F, Ishida Y, Hotta K, et al. Autophosphorylation of insulin receptor in a patient with Werner's syndrome associated with insulin resistant diabetes mellitus.. Endocr J. 1995; 42(1):107-13. (Clone-specific: ELISA). View Reference
  6. Soos MA, O'Brien RM, Brindle NP, et al. Monoclonal antibodies to the insulin receptor mimic metabolic effects of insulin but do not stimulate receptor autophosphorylation in transfected NIH 3T3 fibroblasts.. Proc Natl Acad Sci USA. 1989; 86(14):5217-21. (Biology). View Reference
  7. Soos MA, Siddle K, Baron MD, et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor.. Biochem J. 1986; 235(1):199-208. (Biology). View Reference
View All (7) View Less
750653 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.