The 455807 monoclonal antibody specifically recognizes CD151, which is also known as Platelet-Endothelial Tetraspan Antigen 3 (PETA-3). CD151 is a member of the Tetraspanin or Transmembrane 4 superfamily (TM4SF). Like most tetraspaninins, CD151 regulates cell migration, interactions, and signaling by forming complexes with other membrane molecules, such as integrins and other tetraspanins. CD151 is expressed on many types of cells, including endothelial and epithelial cells, megakaryocytes and platelets, but not on lymphoid cells.
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).