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      Biotin Mouse Anti-Rat IgG2a
      Product Details
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      BD Pharmingen™
      Rat (QC Testing)
      Mouse SJL IgG2b, κ
      Rat Pooled IgG
      ELISA Detection (Routinely Tested), Flow cytometry (Tested During Development)
      0.5 mg/ml
      AB_395122
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      For the sandwich rat IgG2a ELISA, biotinylated mAb RG7/1.30 is optimal for detection with purified anti-rat IgG2a mAb B46-7 (Cat. No. 553918) for capture. Biotin-conjugated RG7/1.30 mAb may be used as a secondary reagent in immunofluorescent staining. RG7/1.30 antibody is effective for detection of cell-surface or intracellular Ig by immunofluorescent staining with flow cytometric analysis. For flow cytometric detection of intracytoplasmic Ig2a, we recommend FITC-conjugated RG7/1.30 mAb (Cat. No. 553896). For immunohistochemical staining, we recommend the use of biotinylated anti-rat IgG1/2a mAb G28-5 in our special formulation for immunohistochemistry, Cat. No. 550325.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      553894 Rev. 10
      Antibody Details
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      RG7/1.30

      The Rg7/1.30 antibody reacts specifically with the Fc region of rat IgG2a. It does not react with other Ig isotypes.

      553894 Rev. 10
      Format Details
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      Biotin
      Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
      Biotin
      553894 Rev.10
      Citations & References
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      Development References (1)

      1. Springer TA, Bhattacharya A, Cardoza JT, Sanchez-Madrid F. Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies. Hybridoma. 1982; 1(3):257-273. (Immunogen). View Reference
      553894 Rev. 10

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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