Alexa Fluor™ 647 Biosimilar Anti-Human CD52

BD Pharmingen™ Alexa Fluor™ 647 Biosimilar Anti-Human CD52

Name: Alexa Fluor™ 647 Biosimilar Anti-Human CD52
Brand: BD Pharmingen™
SKU: 571184

Clone Alemtuzumab297.rMAb (also known as Alemtuzumab N297A Biosimilar.rMAb)

(RUO)

Multiparameter flow cytometric analysis of CD52 (Alemtuzumab Biosimilar) expression on Human peripheral blood leukocyte populations. Human whole blood was stained with either Alexa Fluor™ 647 Human IgG1, κ Isotype Control (Cat. No. 569961; Left Plot) or Alexa Fluor™ 647 Biosimilar Anti-Human CD52 antibody (Cat. No. 571184/571185; Right Plot). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). The bivariate pseudocolor density plot showing the correlated expression of CD52 (Alemtuzumab Biosimilar) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.

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Product Details

Brand: BD Pharmingen™

Alternative Name: CAMPATH-1 antigen; HE5

Reactivity: Human (QC Testing)

Isotype: Human IgG1, κ

Immunogen: Human CD52

Application: Flow cytometry (Routinely Tested)

Vol. Per Test: 5 µl/test

Entrez Gene ID: 1043

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
An isotype control should be used at the same concentration as the antibody of interest.
This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Alexa Fluor™ is a trademark of Life Technologies Corporation.
For U.S. patents that may apply, see bd.com/patents.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Lysing Solution 10X Concentrate IVD

Size 100 mL Cat No. 349202

Alexa Fluor™ 647 IgG1, κ N297A Human Isotype Control RUO

Size 0.1 mg Cat No. 569961

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Antibody Details

Alemtuzumab297.rMAb

The Alemtuzumab297.rMAb (also known as, Alemtuzumab N297A Biosimilar.rMAb) is a research grade humanized recombinant human IgG1, kappa antibody that specifically recognizes the extracellular domain of human CD52, similarly to the therapeutic Alemtuzumab antibody. The Alemtuzumab297.rMAb uses variable region sequences from Alemtuzumab and constant region consensus sequences from human IgG1 kappa. The asparagine at position 297 of the constant heavy chain has been replaced with alanine (N297A) to further reduce Fc receptor interactions. The CD52 antigen is a 25-29 kDa N-glycosylated, GPI-linked protein that is also known as Cambridge pathology antigen 1 (CAMPATH-1 antigen), or Human epididymis-specific protein 5 (He5). It is expressed on thymocytes, lymphocytes, monocytes, macrophages, dendritic cells, and eosinophils, but is weakly or not expressed on neutrophils, erythrocytes, platelets, or hematopoietic stem cells. CD52 is variably expressed on malignant lymphoid cells and serves as a target for therapeutic CD52-specific antibodies.

The Alemtuzumab297.rMAb is intended for research use only. It is not intended for use in therapeutic or diagnostic procedures for humans or animals.

571184 Rev. 1

Format Details

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: Alexa Fluor™ 647

Excitation Source: Red 627-640 nm

Excitation Max: 653 nm

Emission Max: 669 nm

571184 Rev.1

Citations & References

View product citations for antibody "571184" on CiteAb

Development References (10)

Bologna L, Gotti E, Manganini M, et al. Mechanism of action of type II, glycoengineered, anti-CD20 monoclonal antibody GA101 in B-chronic lymphocytic leukemia whole blood assays in comparison with rituximab and alemtuzumab.. J Immunol. 2011; 186(6):3762-9. (Biology). View Reference
Golay J, Manganini M, Rambaldi A, Introna M. Effect of alemtuzumab on neoplastic B cells.. Haematologica. 2004; 89(12):1476-83. (Biology). View Reference
Hale G. Synthetic peptide mimotope of the CAMPATH-1 (CD52) antigen, a small glycosylphosphatidylinositol-anchored glycoprotein.. Immunotechnology. 1995; 1(3-4):175-87. (Biology). View Reference
Havari E, Turner MJ, Campos-Rivera J, et al. Impact of alemtuzumab treatment on the survival and function of human regulatory T cells in vitro.. Immunology. 2014; 141(1):123-31. (Biology). View Reference
Lowenstein H, Shah A, Chant A, Khan A. Different mechanisms of Campath-1H-mediated depletion for CD4 and CD8 T cells in peripheral blood.. Transpl Int. 2006; 19(11):927-36. (Biology). View Reference
Mohan SR, Clemente MJ, Afable M, et al. Therapeutic implications of variable expression of CD52 on clonal cytotoxic T cells in CD8+ large granular lymphocyte leukemia.. Haematologica. 2009; 94(10):1407-14. (Biology). View Reference
Pernick N. CD50-59. 2023. Available: https://www.pathologyoutlines.com/topic/cdmarkerscd50.html 10/162023.
Riechmann V, van Crüchten I, Sablitzky F. The expression pattern of Id4, a novel dominant negative helix-loop-helix protein, is distinct from Id1, Id2 and Id3. Nucleic Acids Res. 1994; 22(5):749-755. (Biology). View Reference
Toh BH, Kyaw T, Tipping P, Bobik A. Immune regulation by CD52-expressing CD4 T cells.. Cell Mol Immunol. 2013; 10(5):379-82. (Biology). View Reference
Trzonkowski P, Zilvetti M, Friend P, Wood KJ. Recipient memory-like lymphocytes remain unresponsive to graft antigens after CAMPATH-1H induction with reduced maintenance immunosuppression.. Transplantation. 2006; 82(10):1342-51. (Biology). View Reference

View All (10)

571184 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.