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Human Monocyte/NK Cell Activation Kit

BD Phosflow™ Human Monocyte/NK Cell Activation Kit

Product Details
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BD Phosflow™
Human (QC Testing)

Preparation And Storage

Store the fluorescent antibodies, BD CompBeads, and Stain Buffer at 2 to 8°C. Do not freeze. The fluorescent antibodies and BD CompBeads should be protected from prolonged exposure to light. Store the Perm Buffer IV, Treated and Untreated Control Cells, and the Lyse/Fix Buffer at room temperature.

562089 Rev. 1
Citations & References
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Development References (10)

  1. Covey TM, Putta S, Cesano A. Single cell network profiling (SCNP): mapping drug and target interactions. Assay Drug Dev Technol. 2010; 8:321–343. (Biology).
  2. Critchley-Thorne RJ, Simons DL, Yan N, et al. Impaired interferon signaling is a common immune defect in human cancer. Proc Natl Acad Sci U S A. 2009; 106:9010–9015. (Biology).
  3. Irish JM, Kotecha N, Nolan GP. Mapping normal and cancer cell signalling networks: towards single-cell proteomics. Nat Rev Cancer. 2006; 6:146-155. (Biology).
  4. Krutzik PO, Crane JM, Clutter MR, Nolan GP. High-content single-cell drug screening with phosphospecific flow cytometry. Nat Chem Biol. 2008; 4(2):132-142. (Biology). View Reference
  5. Lee AW, Sharp ER, O'Mahony A, et al. Single-cell, phosphoepitope-specific analysis demonstrates cell type- and pathway-specific dysregulation of Jak/STAT and MAPK signaling associated with in vivo human immunodeficiency virus type 1 infection. J Virol. 2008; 82:3702–3712. (Biology).
  6. Montag DT, Lotze MT. Rapid flow cytometric measurement of cytokine-induced phosphorylation pathways [CIPP] in human peripheral blood leukocytes. Clin Immunol. 2006; 121(2):215-226. (Biology). View Reference
  7. Montag DT, Lotze MT. Successful simultaneous measurement of cell membrane and cytokine induced phosphorylation pathways [CIPP] in human peripheral blood mononuclear cells. J Immunol Methods. 2006; 313(1-2):48-60. (Biology). View Reference
  8. Perez OD, Krutzik PO, Nolan GP. Flow cytometric analysis of kinase signaling cascades. Methods Mol Biol. 2004; 263:67–94. (Biology).
  9. Perez OD, Mitchell D, Campos R, Gao GJ, Li L, Nolan GP. Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry. Curr Protoc Cytom. 2005; 6.20.1-6.20.22. (Biology). View Reference
  10. Schulz KR, Danna EA, Krutzik PO, Nolan GP. Single-cell phospho-protein analysis by flow cytometry. Curr Protoc Immunol. 2007. (Biology).
View All (10) View Less
562089 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.