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Purified Mouse Anti-Human CD3
Product Details
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BD™
CD3-epsilon; CD3E; Leu4; T-cell surface antigen T3/Leu-4 epsilon chain; T3E
Human
Mouse BALB/c IgG1, κ
Human Thymocytes
Flow cytometry
6.25 μg/mL
20 μL
II T118; III T492
916
AB_400284
Phosphate buffered saline with gelatin 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347340 Rev. 1
Antibody Details
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SK7

The CD3 antibody, clone SK7, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with human thymocytes.

The CD3 antibody reacts with the epsilon chain of the CD3 antigen/TCR complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kilodaltons (kDa). The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).

347340 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
347340 Rev.1
Citations & References
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View product citations for antibody "347340" on CiteAb

Development References (1)

  1. Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983; 131:536-539. (Biology).
347340 Rev. 1

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