Name: Purified Mouse Anti-CLAMP
Brand: BD Transduction Laboratories™
SKU: 612660

Purified Mouse Anti-CLAMP

Clone 42/CLAMP

(RUO)

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Protocol Library

Scientific Resources

Western blot analysis of CLAMP on rat kidney lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-CLAMP.

Protocol Library

Scientific Resources

Product Details

Brand: BD Transduction Laboratories™

Isotype: Mouse IgG1

Immunogen: Rat CLAMP aa. 431-523

Application: Western blot (Routinely Tested)

Target Molecular Weight: 74 kDa

Concentration: 250 µg/ml

RRID: AB_399904

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

612660 Rev. 1

Antibody Details

42/CLAMP

PDZ domain containing proteins are involved in protein-protein interactions in various cell functions, such as receptor recycling, synaptic transmission, and cytoskeletal dynamics. PDZ domains form a β-sandwich with the hydrophobic pocket of C-terminal PDZ-interacting domains. CLAMP is a PDZ domain-containing protein that is also known as PDZK1, Diphor-1, CAP70, and NaPi-Cap1. It contains four PDZ domains and interacts with the C-terminus of Scavenger receptor B type I (SR-BI). The latter regulates SR-BI surface expression and reverses cholesterol transport in the liver. CLAMP mRNA is expressed in kidney, liver, pancreas, gastrointestinal tract, and adrenal cortex. Thus, CLAMP interaction with a variety of cell surface receptors may regulate their expression and function.

612660 Rev. 1

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

612660 Rev.1

Citations & References

Development References (3)

Ikemoto M, Arai H, Feng D, et al. Identification of a PDZ-domain-containing protein that interacts with the scavenger receptor class B type I. Proc Natl Acad Sci U S A. 2000; 97(12):6538-6543. (Biology). View Reference
Kocher O, Comella N, Tognazzi K, Brown LF. Identification and partial characterization of PDZK1: a novel protein containing PDZ interaction domains. Lab Invest. 1998; 78(1):117-125. (Biology). View Reference
Silver DL. A carboxyl-terminal PDZ-interacting domain of scavenger receptor B, type I is essential for cell surface expression in liver. J Biol Chem. 2002; 277(37):34042-34047. (Biology). View Reference

612660 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.