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Purified Mouse Anti-SKAP55
Product Details
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BD Transduction Laboratories™
Rat (QC Testing), Human (Tested in Development)
Mouse IgG1
Human SKAP55 aa. 154-353
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
55 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611236 Rev. 1
Antibody Details
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Immediately following T cell receptor (TcR) ligation, cytoplasmic protein-tyrosine kinases (PTKs) are activated, resulting in the phosphorylation of intracellular and transmembrane proteins. p56[lck] and p59[fyn] are two Src family PTKs activated by TcR ligation. Following activation, Fyn interacts with several tyrosine-phosphorylated proteins, including SKAP55 (Src kinase-associated phosphoprotein of 55 kDa). SKAP55, which selectively binds Src kinase SH2 domains, contains a pleckstrin homology (PH) domain, a C-terminal Src homology 3 (SH3) domain, and multiple tyrosine phosphorylation sites. In addition, SKAP55 directly binds to FYB/SLAP-130, a novel substrate of TcR-stimulated protein tyrosine kinases. Like SLAP-130, SKAP55 is expressed exclusively in mononuclear cells, with preferential expression in T lymphocytes where it is constitutively phosphorylated in resting cells. A SKAP55 homolog, SKAP-HOM, has been identified which also interacts with SLAP-130, exhibits ubiquitous expression, and is phosphorylated only following T cell activation. Therefore, the Fyn-associated protein, SKAP55, along with SLAP-130, are components of a Fyn-mediated signaling cascade in T cells.

611236 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
611236 Rev.1
Citations & References
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Development References (5)

  1. Liu J, Kang H, Raab M, da Silva AJ, Kraeft SK, Rudd CE. FYB (FYN binding protein) serves as a binding partner for lymphoid protein and FYN kinase substrate SKAP55 and a SKAP55-related protein in T cells. Proc Natl Acad Sci U S A. 1998; 95(15):8779-8784. (Biology). View Reference
  2. Marie-Cardine A, Bruyns E, Eckerskorn C, Kirchgessner H, Meuer SC, Schraven B. Molecular cloning of SKAP55, a novel protein that associates with the protein tyrosine kinase p59fyn in human T-lymphocytes. J Biol Chem. 1997; 272(26):16077-16080. (Biology). View Reference
  3. Marie-Cardine A, Verhagen AM, Eckerskorn C, Schraven B. SKAP-HOM, a novel adaptor protein homologous to the FYN-associated protein SKAP55. FEBS Lett. 1998; 435(1):55-60. (Biology). View Reference
  4. Wu L, Fu J, Shen SH. SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription. Mol Biol Cell. 2002; 22(8):2673-2686. (Clone-specific: Immunoprecipitation, Western blot). View Reference
  5. Wu L, Yu Z, Shen SH. SKAP55 recruits to lipid rafts and positively mediates the MAPK pathway upon T cell receptor activation. J Biol Chem. 2002; 277(43):40420-40427. (Clone-specific: Immunoprecipitation, Western blot). View Reference
View All (5) View Less
611236 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.