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Purified Mouse Anti-Rho
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human Rho aa. 1-155
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
21 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610990 Rev. 2
Antibody Details
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Members of the Ras superfamily of GTPases are distantly related to the heterotrimeric G proteins and shuttle between inactive and active GTP-bound forms. Interconversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The subfamilies of the Ras-like proteins share 30-50% homology, each member participates in a particular set of cellular functions. The Rho (Ras homolog) proteins (A, B, & C) are highly homologous and contain the consensus amino acid sequences necessary for GDP/GTP-binding and GTPase activity. Rho proteins contain a C-terminal sequence, CAAL, whose post-translational modification regulates Rho activation and function. Rho proteins modulate the in vitro activity of PI-3-kinase, PI- 4,5-kinase, and phospholipase D. Rho is a molecular switch that regulates cell morphology and motility in response to extracellular signals. Due to sequence homology among various small GTPase family members, potential cross-reactivity could be observed with this antibody. Based on recent testing in native ELISA with recombinant proteins to CDC42 and Rac1, we have concluded that this antibody recognizes CDC42 and Rac1. BD Pharmingen has not performed such cross-reactivity testing on other family members.

610990 Rev. 2
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610990 Rev.2
Citations & References
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Development References (5)

  1. Brandt D, Gimona M, Hillmann M, Haller H, Mischak H. Protein kinase C induces actin reorganization via a Src- and Rho-dependent pathway. J Biol Chem. 2002; 277(23):20903-20910. (Clone-specific: Western blot). View Reference
  2. Madaule P, Axel R. A novel ras-related gene family. Cell. 1985; 41(1):31-40. (Biology). View Reference
  3. Maddox AS, Burridge K. RhoA is required for cortical retraction and rigidity during mitotic cell rounding. J Cell Biol. 2003; 160(2):255-265. (Clone-specific: Western blot). View Reference
  4. Takai Y, Sasaki T, Tanaka K, Nakanishi H. Rho as a regulator of the cytoskeleton. Trends Biochem Sci. 1995; 20(6):227-231. (Biology). View Reference
  5. Wenk MB, Midwood KS, Schwarzbauer JE. Tenascin-C suppresses Rho activation. J Cell Biol. 2000; 150(4):913-920. (Clone-specific: Western blot). View Reference
View All (5) View Less
610990 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.