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Purified Mouse Anti-Phosphotyrosine
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Purified Mouse Anti-Phosphotyrosine
Western blot analysis of Phosphotyrosine on A431 lysate. 1:1000 (lane 1), 1:2000 (lane 2), 1:4000 (lane 3) dilution of Phosphotyrosine.
Purified Mouse Anti-Phosphotyrosine

Phosphotyrosine, clone PY69. Left panel: A431 control. Right panel: A431 + EGF

Western blot analysis of Phosphotyrosine on A431 lysate. 1:1000 (lane 1), 1:2000 (lane 2), 1:4000 (lane 3) dilution of Phosphotyrosine.

Phosphotyrosine, clone PY69. Left panel: A431 control. Right panel: A431 + EGF

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Chicken, Dog, Frog (Tested in Development)
Mouse IgG2a
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
1.0 mg/ml
Aqueous buffered solution containing glycerol and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610430 Rev. 1
Antibody Details
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Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins.

610430 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610430 Rev.1
Citations & References
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Development References (5)

  1. Doronin S, Shumay E, Wang HY, Malbon CC. Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin. J Biol Chem. 2002; 277(17):15124-15131. (Clone-specific: Western blot). View Reference
  2. Doronin S, Wang Hy HY, Malbon CC. Insulin stimulates phosphorylation of the beta 2-adrenergic receptor by the insulin receptor, creating a potent feedback inhibitor of its tyrosine kinase. J Biol Chem. 2002; 277(12):10698-10703. (Clone-specific: Western blot). View Reference
  3. Glenney JR Jr, Zokas L, Kamps MP. Monoclonal antibodies to phosphotyrosine. J Immunol Methods. 1988; 109(2):277-285. (Biology). View Reference
  4. Moro L, Dolce L, Cabodi S, et al. Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p13Cas and leads to phosphorylation of specific EGF receptor tyrosines. J Biol Chem. 2002; 277(11):9405-9414. (Clone-specific: Western blot).
  5. Yan Z, Deng X, Friedman E. Oncogenic Ki-ras confers a more aggressive colon cancer phenotype through modification of transforming growth factor-beta receptor III. J Biol Chem. 2001; 276(2):1555-1563. (Clone-specific: Western blot). View Reference
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610430 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.