The cerebral cortex is composed of multiple layers of neurons of distinct types and functions, which perform in unison to orchestrate cognitive function. The formation of the cortex relies on complex signaling mechanisms, which mediate the migration of newly formed neurons from deep within the brain to the superficial regions. Defects in neuronal migration and disruption of the multi-layered cortex are apparent in conditions such as X-linked lissencephaly (XLIS) and subcortical laminar heterotopia (SCLH) or "double cortex" (DC) syndrome. Mutations in the doublecortin gene have been linked to these brain disorders. Doublecortin is highly expressed in developing brain, primarily in migrating neurons. It is significantly homologous with the N-terminal region of the product of the KIAA0369 gene, a protein that is also similar to the CaM kinase family in its C-terminal region. Doublecortin has four potential MAP kinase family phosphorylation sites and a putative site for Abl tyrosine phosphorylation. Thus, doublecortin is thought to be an integral component of tyrosine kinase signal transduction pathways that regulate neuronal migration and development of the cerebral cortex.
The 30/Doublecortin monoclonal antibody reacts with mouse and human doublecortin.