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Purified Mouse Anti-Pig IgG
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This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
Product Details
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BD Pharmingen™
Pig (QC Testing)
Mouse BALB/c IgG1, κ
Purified pig IgG
ELISA Capture (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

We recommend using the purified F007-1241 mAb for capture in sandwich pig IgG ELISA in combination with biotinylated anti-pig IgG F007-1241 mAb (Cat. No. 552555) for detection.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
552554 Rev. 2
Antibody Details
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The F007-1241 antibody reacts with pig IgG, but not with pig IgM. The reactivity with individual pig IgG subclasses (IgG1, IgG2a, IgG2b, IgG3, IgG4) was not determined. It does not cross-react with mouse, rat, hamster, or rabbit immunoglobulins. The F007-1241 antibody does have some cross-reactivity with dog immunoglobulins.

552554 Rev. 2
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
552554 Rev.2
Citations & References
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View product citations for antibody "552554" on CiteAb
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No Citations are available for this Product

552554 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.