Purified Mouse Anti-Human CD121a (IL1-RI)

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BD Pharmingen™ Purified Mouse Anti-Human CD121a (IL1-RI)

Name: Purified Mouse Anti-Human CD121a (IL1-RI)
Brand: BD Pharmingen™
SKU: 551388

Clone hIL1R-M1

(RUO)

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Product Details

Brand: BD Pharmingen™

Alternative Name: IL-1 Receptor Type 1

Reactivity: Human (QC Testing)

Isotype: Mouse IgG1, κ

Application: ELISA (Routinely Tested), Western blot (Reported)

Concentration: 0.5 mg/ml

RRID: AB_2264756

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

ELISA Capture: Purified Mouse Anti-Human CD121a (IL1-RI) (Cat. No. 551388) is useful as a capture antibody for a sandwich ELISA for measuring human IL-1 receptor I protein levels. Purified Mouse Anti-Human CD121a (IL1-RI) can be paired with a biotinylated detecting antibody, and recombinant soluble human IL-1 receptor type I as the standard. Purified Mouse Anti-Human CD121a (IL1-RI) should be titrated between 1 - 4 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant human IL-1 receptor type I ranging from 3000 to 20 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section under "ELISA and ELISPOT" at our website: http://www.bdbiosciences.com/us/s/resources.

Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines: sCD14, CD40, CD40L, IL-1Ra, IL-1RII, IL-1α, IL-1β, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-6R, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF, LT-α, sTNFRI, sTNFRII, IFNγ, TRAIL, GM-CSF, TGFβ, Trx

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

551388 Rev. 2

Antibody Details

hIL1R-M1

The HIL1R-M1 antibody reacts with recombinant and natural soluble CD121a which is also known as human IL-1 receptor type I proteins. Human IL-1 receptor type I serves as the ligand-binding chain of the IL-1 heterodimer complex. When produced by proteolytic cleavage, soluble forms of the IL-1R type I are found the circulation of healthy individuals and in excessive levels during inflammatory diseases. The human IL-1 receptor type I is also expressed predominantly by T lymphocytes, fibroblasts, and endothelial cells.

551388 Rev. 2

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

551388 Rev.2

Citations & References

Development References (5)

Bernstein SH, Fay J, Frankel S, et al. A phase I study of recombinant human soluble interleukin-1 receptor (rhu IL-1R) in patients with relapsed and refractory acute myeloid leukemia. Cancer Chemother Pharmacol. 1999; 43(2):141-144. (Biology). View Reference
Born TL, Smith DE, Garka KE, Renshaw BR, Bertles JS, Sims JE. Identification and characterization of two members of a novel class of the interleukin-1 receptor (IL-1R) family. Delineation of a new class of IL-1R-related proteins based on signaling. J Biol Chem. 2000; 275(39):29946-29954. (Biology). View Reference
Sims JE, Giri JG, Dower SK. The two interleukin-1 receptors play different roles in IL-1 actions. Clin Immunol Immunopathol. 1994; 72(1):9-14. (Biology). View Reference
Sims JE, Painter SL, Gow IR. Genomic organization of the type I and type II IL-1 receptors. Cytokine. 1995; 7(6):483-490. (Biology). View Reference
Sims JE. CD121a. J Biol Regul Homeost Agents. 2000; 14(2):133-135. (Biology). View Reference

View All (5)

551388 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.