Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
France (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      RY586 Rat Anti-Mouse CD26
      Product Details
      Down Arrow Up Arrow


      BD OptiBuild™
      Cd26; Dpp4; dipeptidylpeptidase 4; THAM, DPP IV
      Mouse (Tested in Development)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
      Cell-surface molecules solubilized from BALB/c mouse thymocytes
      Flow cytometry (Qualified)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Researchers should determine the optimal concentration of this reagent for their individual applications.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. CF™ is a trademark of Biotium, Inc.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      Show More blue down arrow Show Less blue up arrow
      753485 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      H194-112

      The H194-112 monoclonal antibody specifically binds to CD26, which is also known as, Thymocyte-activating molecule (THAM), or dipeptidyl peptidase IV (DPP IV, Dpp4).  CD26 is a ~220- kDa dimer formed of identical type-II transmembrane core polypeptides which undergo variable post-translational modifications.  It is a multi-functional molecule with both ectopeptidase and signal-transducing activities.  Studies with specific DPP IV inhibitors suggest that the enzymatic activity is involved in the mediation of T-cell activation events.  The expression of CD26 is developmentally regulated in the thymus.  Resting lymphoid cells of the bone marrow and peripheral B and T lymphocytes express low levels of CD26; bone-marrow and peritoneal myeloid cells do not.  CD26 is also found on epithelial cells in the kidney, liver, small intestine, and lung.  Cross-linked H194-112 mAb induces proliferation of immature and mature thymocytes in the presence of either IL-1 plus IL-2 or PMA;  addition of IL-2 or IL-4 to PMA further enhances the activation.

      753485 Rev. 1
      Format Details
      Down Arrow Up Arrow
      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
      altImg
      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      753485 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "753485" on CiteAb

      Development References (7)

      1. Fleischer B. CD26: a surface protease involved in T-cell activation. Immunol Today. 1994; 15(4):180-184. (Biology). View Reference
      2. Gorvel JP, Vivier I, Naquet P, Brekelmans P, Rigal A, Pierres M. Characterization of the neutral aminopeptidase activity associated to the mouse thymocyte-activating molecule. J Immunol. 1990; 144(8):2899-2907. (Biology). View Reference
      3. Marguet D, Bernard AM, Vivier I, Darmoul D, Naquet P, Pierres M. cDNA cloning for mouse thymocyte-activating molecule. A multifunctional ecto-dipeptidyl peptidase IV (CD26) included in a subgroup of serine proteases. J Biol Chem. 1992; 267(4):2200-2208. (Biology). View Reference
      4. Naquet P, MacDonald HR, Brekelmans P. A novel T cell-activating molecule (THAM) highly expressed on CD4-CD8- murine thymocytes. J Immunol. 1988; 141(12):4101-4109. (Immunogen: Activation). View Reference
      5. Naquet P, Vivier I, Gorvel JP. Activation of mouse T lymphocytes by a monoclonal antibody to a developmentally regulated surface aminopeptidase (THAM). Immunol Rev. 1989; 111:177-193. (Clone-specific: Activation). View Reference
      6. Reinhold D, Bank U, Buhling F. Inhibitors of dipeptidyl peptidase IV (DP IV, CD26) induces secretion of transforming growth factor-beta 1 (TGF-beta 1) in stimulated mouse splenocytes and thymocytes. Immunol Lett. 1997; 58(1):29-35. (Biology: Activation). View Reference
      7. Vivier I, Marguet D, Naquet P . Evidence that thymocyte-activating molecule is mouse CD26 (dipeptidyl peptidase IV). J Immunol. 1988; 147(2):447-454. (Biology). View Reference
      View All (7) View Less
      753485 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.