RY586 Rat Anti-Mouse CD16/CD32

Try the new Anti-Mouse CD16/CD32 antibody: 2.4G2

BD OptiBuild™ RY586 Rat Anti-Mouse CD16/CD32

Name: RY586 Rat Anti-Mouse CD16/CD32
Brand: BD OptiBuild™
SKU: 753655

Clone 190909

(RUO)

Product Details

Brand: BD OptiBuild™

Alternative Name: Fcgr3/Fcgr2b; Fc gamma RIII/Fc gamma RIIB; FcγRIII/FcγRIIB

Reactivity: Mouse (Tested in Development)

Isotype: Rat IgG2b, κ

Immunogen: Mouse Fc gamma RIIB Recombinant Protein

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Researchers should determine the optimal concentration of this reagent for their individual applications.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
CF™ is a trademark of Biotium, Inc.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 mL Cat No. 554657

Lysing Buffer RUO

Size 100 mL Cat No. 555899

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.5 mg Cat No. 553142

RY586 Rat IgG2b, κ Isotype Control RUO

Size 50 µg Cat No. 568160

753655 Rev. 1

Antibody Details

190909

The 190909 antibody specifically recognizes a common epitope on the extracellular domains of CD16 (also known as, Fc gamma RIII) and CD32 (Fc gamma RII). CD16 and CD32 serve as low affinity receptors for mouse IgG Fc constant regions and play roles in the activation or inhibition of phagocytosis, antibody-dependent cellular cytotoxicity (ADCC), degranulation, and B cell proliferation. These receptors are variably expressed on B lymphocytes, natural killer (NK) cells, monocytes, macrophages, dendritic cells, Kupffer cells, granulocytes, mast cells, immature thymocytes, and some activated mature T lymphocytes. CD16 and CD32 are single-pass type I transmembrane glycoproteins with two extracellular Ig-like domains and belong to the Ig superfamily. Ligand-bound CD16 can associate with two disulfide-linked FcR-gamma subunits (FcεRIγ) that contain cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) which deliver activating signals intracellularly. CD32 has an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and functions as an inhibitory receptor.

753655 Rev. 1

Format Details

RY586

The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.

Format: RY586

Excitation Source: Yellow-Green 561 nm

Excitation Max: 564 nm

Emission Max: 586 nm

753655 Rev.1

Citations & References

View product citations for antibody "753655" on CiteAb

Development References (2)

Kool M, Geurtsvankessel C, Muskens F, et al. Facilitated antigen uptake and timed exposure to TLR ligands dictate the antigen-presenting potential of plasmacytoid DCs.. J Leukoc Biol. 2011; 90(6):1177-90. (Clone-specific: Flow cytometry). View Reference
Nimmerjahn F, Ravetch JV. FcγRs in health and disease. Curr Top Microbiol Immunol. 2011; 350:105-125. (Biology). View Reference

753655 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.