BD Horizon™ RB780 Rat Anti-Mouse CD8b
Clone YTS156.7.7.rMAb (also known as YTS156.7.7; YTS156)
(RUO)
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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
The YTS156.7.7.rMAb is a recombinant monoclonal antibody derived from YTS156.7.7 hybridoma cells that specifically recognizes the β chain of the CD8 differentiation antigen (CD8b), which is also known as Lymphocyte antigen 3 (Ly-3) or Lyt-3. CD8b is a single-pass type I transmembrane glycoprotein that is encoded by Cd8b1 (CD8 antigen, beta chain 1) which belongs to the Ig superfamily. CD8b is comprised of an N-terminal IgV domain in its extracellular region followed by a transmembrane region and a cytoplasmic tail. CD8b can bind to the CD8 α chain (CD8a) to form the disulfide-linked CD8 αβ (CD8ab) heterodimer also known as Ly2,3 or Lyt-2,3. This heterodimer is expressed on most thymocytes and a subpopulation of MHC class I-restricted mature T cells. CD8 αβ functions as an antigen coreceptor on the T-cell surface which interacts with MHC class I molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and the protein tyrosine kinase, lck (p56lck).
Development References (5)
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Cobbold S, Martin G, Waldmann H. Monoclonal antibodies for the prevention of graft-versus-host disease and marrow graft rejection. The depletion of T cell subsets in vitro and in vivo.. Transplantation. 1986; 42(3):239-47. (Immunogen: Flow cytometry). View Reference
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Liunggren G, Liunggren HG, Dalianis T. T cell subsets involved in immunity against polyoma virus-induced tumors.. Virology. 1994; 198(2):714-6. (Clone-specific: In vivo exacerbation). View Reference
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Monney L1, Sabatos CA, Gaglia JL, et al. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease.. Nature. 2002; 415(6871):536-541. (Clone-specific: Flow cytometry). View Reference
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Shore DA, Issafras H, Landais E, Teyton L, Wilson IA. The crystal structure of CD8 in complex with YTS156.7.7 Fab and interaction with other CD8 antibodies define the binding mode of CD8 alphabeta to MHC class I.. J Mol Biol. 2008; 384(5):1190-202. (Clone-specific: Blocking). View Reference
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Wei Y, Chen K, Sharp GC, Yagita H, Braley-Mullen H. Expression and regulation of Fas and Fas ligand on thyrocytes and infiltrating cells during induction and resolution of granulomatous experimental autoimmune thyroiditis.. J Immunol. 2001; 167(11):6678-86. (Clone-specific: In vivo exacerbation). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.